The present invention discloses a high-GC content of PCR gene amplification buffer. 所述高GC含量基因的PCR扩增缓冲液包括如下组分:TrisHcl150mmol/L,(NH4)2SO440mmol/L,一水甜菜碱2.6mol/L,乳化剂Tween 20 0.02%,PCR反应增强剂20%,配置后,过滤去菌,20℃保存. The GC-rich PCR gene amplification ...
别名: 高GC DNA清除剂,PCR级 高GC DNA清除剂,PCR级(High GC DNA Erasol)提供了更简单、更快速和更高质量的方法,包括质粒提取、胶回收、PCR产物纯化、基因组提取、RNA分离纯化、PCR/RT-PCR、荧光定量、克隆载体和感受态细胞制备等数百种实验常用试剂盒。 高GC DNA清除剂,PCR级(High GC DNA Erasol) 本产品...
该试剂盒已针对多种 RNA 模板进行了广泛验证,包括富含 GC 和 AU 的靶标,以及低水平表达的 RNA。此外,还可通过 cDNA 的体外转录中高效生成 cRNA PCR & 实时荧光定量 PCR、反转录 货号规格反应次数 4368814 Kit only 200 次反应 4368813 Kit only 1,000 次反应 4374966 Kit with Rnase Inhibitor 200 次反应 ...
Plasmid pBBR15.2-2H12.D11opt was constructed in two steps; first, a SpeI on the backbone was eliminated by PCR-amplification of pBBR15-2H12.D11 with oligos 18655/18656 and circularization of the PCR product using Gibson assembly; next, a high-GC-content-optimized variant of 2H12.D11 ...
False-negative and false-positive results can be generated by NGS-based platforms in genomic regions with highly repetitive sequence, high GC content, or homologous sequences. The regions with high GC content or repetitive sequences are often underrepresented in the NGS coverage because of bias in...
Four GC-rich fragments (0.74 kb, 0.58 kb, 0.71 kb, and 0.72 kb in length; GC content indicated above) were amplified from 50 ng of human genomic DNA. The same targets were also amplified using competitor DNA polymerases according to manufacturer recommended protocols for GC-ri...
(6) The content of GC bases for each primer is between 45 and 65%, and the primer should avoid over 4 consecutive G or other bases. Figure 2 Establishment of the method for site-directed mutagenesis of large plasmids (SMLP) based on PCR. (A) A plasmid map showing the locations of ...
Custom index1 sequencing primer (PAGE purified) was added to the machine: 5′- act ctg cgt tga tac cac tgc ttc cgc gga cag gc −3′. Raw reads were first demultiplexed by standard Illumina protocols. The demultiplexed reads were processed with a custom script. Briefly, the first 10 ...
and the UPF280make up the core network, referred to in 5G as the 5G core (5GC), and may be a specific example of the core network170in FIG.1. The various network functions illustrated in FIG.2A may be distributed over separate devices (e.g., servers), or may be logically separated...
CNVkit (v0.9.6) was used to quantify read abundance in genomic window of 200 kb, and normalized for GC content and mappability, excluding outlier bins. Segmentation was performed using a three state HMM for amplified and deleted regions (Talevich et al., 2016)....