A+T content = 29.04% Nucleotide Number Mol% A 173 14.27 C 438 36.14 G 422 34.82 T 179 14.77 解决: 1.由于基因中GC含量过高,就不应该用普通的buffer了,而应该用特殊的---GC buffer,对于TM值可以做一下梯度PCR,GC buffer可以在一些公司都能买到。买TAKALA的GC buffer for PCR.还有,设计一段合适的引...
double stranded G+C content = 70.96% A+T content = 29.04% Nucleotide Number Mol% A 173 14.27 C 438 36.14 G 422 34.82 T 179 14.77 解决: 1.由于基因中 GC 含量过高,就不应该用普通的 buffer 了,而应该用特殊的---GC buffer ,对于 TM 值可以做一下梯度 PCR,GC buffer 可以在一些公司都能买到...
K. GC Content-Based Pan-Pox Universal PCR Assays for Poxvirus Detection. Journal of Clinical Microbiology 48, 268-276, https://doi.org/10.1128/JCM.01697-09 (2010).Li Y., H. Meyer, H. Zhao, and I. K. Damon. 2010. GC content-based pan-pox universal PCR assays for poxvirus detection...
Fine-tuning of PCR conditions is not practicable for all target sequences whenever a large number of genes of different lengths and GC content are to be amplified in parallel, particularly if total open reading frame or domain coverage is essential for recombinant protein synthesis. Thus, the ...
Applications of GC content When considering a fragment of DNA, for primer design or cloning of an entire gene, it is important to note GC content given the higher amount of energy required to break the three hydrogen bonds. Polymerase chain reaction (PCR) is a widely utilized tool where GC...
A+T content = 29.04% Nucleotide Number Mol% A 173 14.27 C 438 36.14 G 422 34.82 T 179 14.77 解决: 1.由于基因中GC含量过高,就不应该用普通的buffer了,而应该用特殊的---GC buffer,对于TM值可以做一下梯度PCR,GC buffer可以在一些公司都能买到。买TAKALA的GC buffer for PCR.还有,设计一段合适的引...
英文: Ethylene Glycol and 1,2-propanediol Enhanced Polymerase Chain Reaction for Amplification of High GC Content Human DNA 中文: 乙二醇和1,2-丙二醇增强富含GC碱基人类基因组DNA模板的PCR扩增更详细... 英文: Comparing Gene Density and GC Content Between Two Large Genomic Blocks at Short Arm of Chro...
to amplify using conventional PCR techniques due the presence of secondary structures that resist denaturation and prevent efficient primer annealing. Takara Bio offers several DNA polymerases designed to handle such challenging targets, with amplification of templates that contain up to 90% GC content. ...
The GC content affects the secondary structure of mRNA. The GC content affects the annealing temperature for template DNA in PCR experiments. Where to apply the GC Content? The GC Content can be used in 1. primer design for PCR experiments 2. gene design from protein expression 3. mRNA ...
The GC content affects the secondary structure of mRNA. The GC content affects the annealing temperature for template DNA in PCR experiments. Where to apply the GC Content? The GC Content can be used in 1. primer design for PCR experiments 2. gene design from protein expression 3. mRNA ...