产品名称 2×Hieff NGS®高GC多重PCR预混液|2×Hieff NGS® HG Multiplex PCR Master Mix 产品规格 1 mL 用途范围 仅做科研,不用于临床 是否进口 否 加工定制 否 商品货号 13283ES 商品规格 1 mL 生产厂家 翌圣生物科技(上海)股份有限公司 规格编码 13283ES03 最小起订量 1.00 发货地 上海...
2×Hieff® C1P6 High GC Multiplex PCR Master Mix以热启动多重PCR酶制剂与2×扩增缓冲液为组分配置成预混液。本预混液可兼容GC含量在25%-70%左右的多重基因扩增,快速便捷地用于多重PCR反应,具备高均一性、高特异性和高灵敏度的特点。本试剂适用于不同多重PCR需求,可用于凝胶电泳鉴定20对以内不同大小...
ResistancePlus® GC is validated for a range of specimen types and powered by proprietary PlexPCR® technologies demonstrating improved multiplex performance
本产品适用于多重PCR实验。2×Hieff NGS® HG Multiplex PCR Master Mix 以热启动多重PCR酶制剂 与 2×扩增缓冲液为组分配置成预混液。本预混液可兼容GC含量在 25%-70%左右的多重基因扩增,快速便捷地用于多重PCR反应,具备高均一性、高特异性和高灵敏度的特点。本试剂适用于不同多重PCR需求, 可用于凝胶...
2.MultiplexPCRamplification.Thepreferentialamplificationoflow-GCcontentsequencesresultsinmisinterpretationofthebalancebetweenthetwosequences.7 •多重PCR•MultiplexPCR 多重PCR(MPCR)或复合PCR，它是在同一 PCR反应体系里加上两对以上引物，同时扩增出多个核酸片段的PCR反应，其反应原理，反应试剂和操作过程与一般PCR...
PCR-direct sequencing of a GC- 86 生物技术通报 Biotechnology Bulletin 2018,Vol.34,No.3 rich region by inclusion of 10% DMSO :application to mouse c-jun[J]. Biotechniques, 1993, 15(3):372. [9]Jensen MA, Fukushima M, Davis RW. DMSO and betaine greatly improve amplification of GC-rich...
目前,研究学者建立了各种不同的方法来解决高GC含量DNA模板扩增困难的问题,包括降落PCR、添加增强剂(如DMSO)等。虽然这些方法和手段的单独使用都获得了一定的效果,但是对于引物和模板的要求普遍过于苛刻,适用范围也比较局限,并不具有普遍适用性,而且往往经过多次摸索和尝试,也不易得到较好的结果。
Development of a simple and type-specific multiplex PCR for Clostridium perfringens; 产气荚膜梭菌型特异性多重PCR的研究 3. Developmentof type and subtype-specific immunoperoxidase staining techniques for detection of avian influenza virus; 禽流感病毒型及亚型特异性免疫酶染色技术的研究 更多例句>> 4...
Samples were tested by VERSANT CT/GC DNA 1.0 Assay (Siemens Healthcare Diagnostics Inc., Tarrytown, NY), a multiplex Real-Time PCR assay, for simultaneous CT/GC detection. Results obtained by VERSANT assay were compared with those obtained by culturing genital secretions of the same patients. ...
Polymerase chain reaction and reverse dot-blot analysis were performed with the Amplitype PM PCR Amplification and Typing Kit (Polymarker Multiplex; Applied Biosystems, Foster City, CA) under conditions recommended by the manufacturer. Estimated allele frequencies in the white sample were in the usual...