Primers for PCR and sequencing should have a GC content between 40 and 60%, with the 3′ of a primer ending in C or G to promote binding. The 3′ end of the primer should be an exact match to the template DNA, because extension by DNA polymerase, during PCR, depends on a good m...
How do surgical techniques affect the clinical outcomes?David Taggart
The instrument specific properties conceived to affect the PCR efficiency are the reaction container, which here in most cases were plastic microtiter well plates, that influences the rate and efficiency of heat transfer, the heating and cooling capacities, which may induce thermal over- and ...
"GC" & "AT" are the probability that a given base is (G or C) or (A or T) (the GC or AT content of the target DNA), "a" is the number of G's and C's and "b" is the number of A's and T's in the restriction enzyme's recognition sequence. For example, for an Ec...
PCR tests for the presence of the actual virus’s genetic material or its fragments as it breaks down. Recently, a group at the University of Oregon investigated the effectiveness of integrated surveillance using an active air sampler13, surface swabs and passive settling plates...
Quantitative PCR was performed on peripheral blood mononuclear cells, which tested positive for pCMV. The patient had hypogammaglobulinemia; therefore, intravenous immunoglobulin was administered, and the antiviral therapy was changed from ganciclovir to cidofovir. The patient was extubated on day 47. ...
(Fig.1). This feature, combined with an unusually high GC content of S-2L genome, explains its exceptionally high melting point12. It is believed that the A-to-Z substitution arose as a form of host evasion tactics, rendering S-2L’s DNA resistant to the DNA-targeting proteins of its ...
Interactions between mitochondrial import components and the respiratory chain can also affect mitochondrial biogenesis. b What are the signals involved in the regulation of mitochondrial biogenesis, in terms of number, size, morphology, protein content and function? These signals may come from outside ...
While it is not possible to accurately simulate how changes in depth will affect RNA-Seq comparative gene expression analyses in all cases, our findings indicate that in diverse species and growth conditions and even with relatively low correlation between biological replicates, 2-3 million fragments...
Finally, sequence variation in the LTR or proviral region of HERV-W is known to affect its transcription [229,230]. However, it is not at all clear that polymorphism rs662139 T/C in a 3-kb region of Xq22.3 (see above) occurs within the LTR or proviral regions of the HERV-W seque...