3.K.Varadaraj.Denaturants or cosolvents improve the specificity of PCR amplification of a GC-richDNA using genetically engineered DNApolymerases. Gene 140, 1–5 (1994).4.Li-Yan Li,et al.A primer designstrategy for PCR amplification of GC-rich DNA sequences.Clinical Biochemistry,2011(44):6...
PCR-amplification of GC-rich regions: ‘slowdown PCR’ Nat Protoc. 2008; 3 :1312–1317. [ PubMed ]Frey UH, Bachmann HS, Peters J, Siffert W. PCR-amplification of GC-rich regions: 'slowdown PCR'. Nature Protocols. 2008; 3 (8):1312–1317. doi: 10.1038/nprot.2008.112. [ Cross Ref ...
PCRAmplificationofHighlyGC-richRegions 高GC含量DNA的PCR扩增 ?1 高GC含量DNA序列扩增困难 生物中广泛存在高GC含量DNA序列,扩增的DNA有的含70%-80%高GC,常规PCR条件下,难扩增,原因:易形成复杂的二级结构,防止DNA模板变性,DNA聚合酶难以结合.2 高GC含量DNA序列扩增常遇到的问题 1.常规PCR.影响PCR产物合成.2.多...
3.K.Varadaraj.Denaturants or cosolvents improve the specificity of PCR amplification of a GC-richDNA using genetically engineered DNApolymerases. Gene 140, 1–5 (1994). 4.Li-Yan Li,et al.A primer designstrategy for PCR amplification of GC-rich DNA sequences.Clinical Biochemistry,2011(44):...
PCR-amplification of GC-rich regions: 'slowdown PCR'. Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification... S Bachmann,J Peters,UH Frey,... 被引量: 0发表: 2007年 Successful amplification of...
Betaine, DMSO, and 7-Deaza-dGTP, a powerful mixture for amplification of GC-rich DNA sequences J Mol Diagn (2006) M. Ralser et al. An efficient and economic enhancer mix for PCR Biochem Biophys Res Commun (2006) A.N. Spiess et al. A highly efficient method for long-chain cDNA synthe...
Long PCR generally refers to amplification of DNA targets longer than 5 kb. Long PCR traditionally has been performed with a blend ofTaqDNA polymerase (for fast elongation) and a high-fidelity enzyme (for accuracy). With the inventions...
Improved PCR method for amplification of GC-rich DNA :对于富含GC的DNA扩增的PCR方法的改进of,GC,p,PCR,for,DNA,rich,PCR扩增 文档格式: .pdf 文档大小: 227.67K 文档页数: 4页 顶/踩数: 0/0 收藏人数: 0 评论次数: 0 文档热度: 文档分类: ...
PCR amplification of highly GC-rich DNA template after denaturation by NaOH 喜欢 0 阅读量: 66 作者:RK Agarwal,A Perl 摘要: Author information: (1)Department of Medicine, State University of New York, Syracuse 13210.关键词:Humans Sodium Hydroxide Guanine Cytosine DNA Polymerase Chain Reaction ...
不像传统的PCR依赖高温解链,RPA/RAA利用功能复合物 (functional complex formed by recombinase & primer)、单链结合蛋白和一些重组反应促进因子来完成解链。(形成D-loop) 所以需要对蛋白酶结构进行优化,使其更适合DNA amplification in GC-rich regions. 我们在该领域做了很多探索,随时欢迎交流探讨。发布...