PCR-amplification of GC-rich regions: ‘slowdown PCR’ Nat Protoc. 2008; 3 :1312–1317. [ PubMed ]Frey UH, Bachmann HS, Peters J, Siffert W. PCR-amplification of GC-rich regions: 'slowdown PCR'. Nature Protocols. 2008; 3 (8):1312–1317. doi: 10.1038/nprot.2008.112. [ Cross Ref ...
PCRAmplificationofHighlyGC-richRegions 高GC含量DNA的PCR扩增 ?1 高GC含量DNA序列扩增困难 生物中广泛存在高GC含量DNA序列,扩增的DNA有的含70%-80%高GC,常规PCR条件下,难扩增,原因:易形成复杂的二级结构,防止DNA模板变性,DNA聚合酶难以结合.2 高GC含量DNA序列扩增常遇到的问题 1.常规PCR.影响PCR产物合成.2.多...
PCR-amplification of GC-rich regions: 'slowdown PCR'. Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification... S Bachmann,J Peters,UH Frey,... 被引量: 0发表: 2007年 Successful amplification of...
Betaine, DMSO, and 7-Deaza-dGTP, a powerful mixture for amplification of GC-rich DNA sequences J Mol Diagn (2006) M. Ralser et al. An efficient and economic enhancer mix for PCR Biochem Biophys Res Commun (2006) A.N. Spiess et al. A highly efficient method for long-chain cDNA synthe...
Figure 6. Amplification of regions of human gDNA with differences in GC content.(A)A ~0.8 kb target with 76% GC was amplified using a DNA polymerase with low processivity. Increasing amounts of DMSO as an additive promote specificity...
Improved PCR method for amplification of GC-rich DNA :对于富含GC的DNA扩增的PCR方法的改进of,GC,p,PCR,for,DNA,rich,PCR扩增 文档格式: .pdf 文档大小: 227.67K 文档页数: 4页 顶/踩数: 0/0 收藏人数: 0 评论次数: 0 文档热度: 文档分类: ...
the non-methylation primers are typically AT-rich and thus need to be long (e.g., >30 nt) with Tm≥60°C to enable specific annealing. In addition, high-AT sequences often favor primer-dimer formation, mismatch hybridization, DNA poly...
PCR amplification of highly GC-rich DNA template after denaturation by NaOH 喜欢 0 阅读量: 66 作者:RK Agarwal,A Perl 摘要: Author information: (1)Department of Medicine, State University of New York, Syracuse 13210.关键词:Humans Sodium Hydroxide Guanine Cytosine DNA Polymerase Chain Reaction ...
PCR amplification of GC-rich DNA regions using the nucleotide analog N4-methyl-2'-deoxycytidine 5'-triphosphate. Authors:Flores-Juárez, Cyntia R and González-Jasso, Eva and Antaramian, Anaid and Pless, Reynaldo C Journal:BioTechniques (2016): 175-182 ...
However, PCR has evolved far beyond simple amplification and detection, and many extensions of the original PCR method have been described. This chapter provides an overview of different types of PCR methods, applications and optimization. Basic PCR The PCR process was originally developed to ...