PCR amplification and sequencing of single copy DNA molecules - Musundi, Soper - 2001 () Citation Context ...ion of interest with high specificity and accuracy (3–8). Robust PCR performance is of the utmost importance for highsensitivity analytical PCR schemes, such as detection of single-...
Wellehan JF Jr, Childress AL, Marschang RE, Johnson AJ, Lamirande EW, Roberts JF, Vickers ML, Gaskin JM, Jacobson ER. Consensus nested PCRamplificationand sequencing of diverse reptilian, avian, and mammalian orthoreoviruses. Vet Microbiol. 2009;133(1-2): 34-42. Doi: 10.1016/j.vetmic.2...
61, No. 3 PCR Ampli?cation and Direct Sequencing of gyrB Genes with Universal Primers and Their Application to the Detection and Taxonomic Analysis of Pseudomonas putida Strains SATOSHI YAMAMOTO* AND SHIGEAKI HARAYAMA Marine Biotechnology Institute, Kamaishi Laboratories, Kamaishi City, Iwate 026, ...
PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas ... Yamamoto,Harayama - 《Applied & Environmental Microbiology》 被引量: 1416发表: 1995年 PCR amplification and direct sequencing of gyrB genes wit...
[6] Rosenthal A, Jones D. Genomic walking and sequencing by oligo-cassette mediatedpolymerase chain reaction. Nucleic Acids Res.1990. 18: 3095-3096. [7] Liu Y, Whittier R. Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from PI and YAC clones...
Enhancing PCR amplification and sequencing using DNA-binding proteins. Mol Biotechnol 2, 295–298 (1994). 16. Hartl, D. L. Molecular melodies in high and low C. Nat Rev Genet 1, 145–149 (2000). 17. Treangen, T. J. & Salzberg, S. L. Repetitive DNA and next-generation sequencing...
The advent of next generation sequencing has coincided with a growth in interest in using these approaches to better understand the role of the structure and function of the microbial communities in human, animal, and environmental health. Yet, use of next generation sequencing to perform 16S rRNA...
Thermostable DNA polymerases used for basic PCR require a DNA template, and as such, the technique is limited to the analysis of DNA samples. Yet numerous instances exist in which amplification of RNA would be preferred. To apply PCR to the study of RNA, the RNA sample must first be conver...
PCR amplification and sequencing success of the four plots in Xishuangbanna.Xiaocui, Huang
[5]Enhancing PCR amplification and sequencing using DNA-binding proteins[J].Ralph Rapley.Molecular Biotechnology.1994(3) [6]纳米材料作为Long PCR增效剂的研究[D].赵鑫.哈尔滨工业大学2011 [7]核内小分子对PCR增效作用的研究[D].赵鹤翔.哈尔滨工业大学2011 ...