Introduction The purpose of this short communication is to de- scribe universal DNA primers for the polymerase chain reaction (PCR) amplification of a 710-bp frag- ment of the mitochondrial cytochrome c oxidase subunit I gene ( COI). This study was motivated by the recent discoveries of more...
Methods in molecular medicineFrey B. Amplification of genomic DNA by PCR. Reischl, U.: Molecular Diagnosis of Infectious Diseases. Totawa, New Jersey: Humana Press, 1998;143-156.Frey, B Amplification of genomic DNA by PCR In: Reischl, U (ed) 1998. Molecular Diagnosis of Infectious Diseases...
这些DNA序列因其高GC含量导致针对它们而设 计的PCR低效或无效扩增现象,必然阻碍了各个领域对针这些基因的相关研究进 程。 目前普遍认为,由高GC含量DNA模板和/或引物的自身互补或其它构象特征 所引起的二级结构,如环状结构或发夹结构等,是导致PCR无效扩增的主要原因。 为了解决高GC含量DNA序列PCR扩增效率低的问题,...
内容提示: CHAPTER 5 DNA Amplification Amplification of DNA may be necessary to increase the quantity of sample available for profiling, to reduce the analysis time, or to produce probes for the hybridization process (Higuchi 1989, Li 1988, Marx 1988, Mullis 1990, Paabo 1989, Saiki 1986). ...
Barnes WM: PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proceedings of the National Academy... WM Barnes - 《Proceedings of the National Academy of Sciences》 被引量: 702发表: 1994年 the fidefity of taq polymerase catalyzing pcr...
REVI&SED UPDATED Edvo-Kit #330 330 PCR Amplification of DNA Experiment Objective: The objective of this experiment is for students to gain hands-on experience of the principles and practice of Polymerase Chain Reaction (PCR). The PCR products are then analyzed by agarose gel electrophoresis. ...
PCR & DNA Amplification Amyloid & Neurodegeneration Stains Cell Cycle Analysis Chromogenic Substrates Equipment Fluorescence Microscopy HRP & Alkaline Phosphatase Conjugates Nuclear Counterstains Nucleic Acid Quantitation & Extraction Super-Resolution Imaging Western & Protein Analysis Antibody & Protein Labeling ...
proteins are coded for by messenger RNA (mRNA), which will later undergo the process of translation. Alternatively, the transcribed gene may encode transfer RNA (tRNA), which carries specificamino acidsto the ribosome for incorporation into the growing protein chain during translation. Another type ...
OPEN SUBJECT AREAS: PCR-BASED TECHNIQUES DNA SEQUENCING Received 28 January 2014 Accepted 6 May 2014 Published 23 May 2014 Correspondence and requests for materials should be addressed to B.U¨ . (ulker@uni-bonn. de) PCR amplification of repetitive DNA: a limitation to genome editing ...
摘要: In this paper we describe a method to amplify mitochondrial and chromosomal DNA from yeasts by PCR using intact cells.关键词: Chromosomes, Fungal Saccharomycetales Saccharomyces cerevisiae Schizosaccharomyces DNA, Mitochondrial Electrophoresis, Agar Gel Polymerase Chain Reaction Base Sequence ...