PCR technology: Principles and applications for DNA amplificationdoi:10.1007/978-1-349-20235-5This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all ...
12月27日,华南农业大学生命科学学院刘耀光院士,陈乐天教授带领的团队,在期刊 Molecular Plant 发表题为 STI PCR: An efficient method for amplification and de novo synthesis of long DNA sequences 的论文。赵哲,谢先荣和刘伟智为该论文共同第一作者。编辑...
Microfluidics: DNA amplification moves on. Nature 2003; 422:28–29; Kricka, L.J., Wilding, P. Microchip PCR. Anal BioAnal Chem 2003; 377:820–825]. In this review, we survey the advances of the above aspects among the PCR microfluidic devices in detail. Finally, we also illuminate the...
The more DNA one has in a sample, the easier it is to identify and diagnose. In many situations, such as old crime scenes or large ecological systems with dilute samples, you want to amplify the amount of available DNA before testing for it. One technique that is most effective in amplif...
Despite continuous improvements,it is difficult to efficiently amplify large sequences from complex templates using current PCR methods.Here,we developed a suppression thermo-interlaced(STI)PCR method for the efficient and specific amplification of long DNA sequences from genomes and synthetic DNA pools....
Thermostable DNA polymerases used for basic PCR require a DNA template, and as such, the technique is limited to the analysis of DNA samples. Yet numerous instances exist in which amplification of RNA would be preferred. To apply PCR to the study of RNA, the RNA sample must first be conver...
Even the use of a non-proofreading but robust DNA polymerase, Taq (NE Biolabs) failed to amplify only the desired fragment (Fig. 1). A laddering of amplification products below the expected size and also a heavy smear above it were observed. Interestingly, the smallest fragment for the ...
Random amplified polymorphic DNA (RAPD) analysis has been applied to the identification of four mussels species: Mytilus edulis, Mytilus chilensis, Mytilus galloprovincialis, and Perna canaliculus. Amplifications of DNA from mussel were carried out using random primers. The most distinctive bands were...
aPCR amplification of target DNA and real-time PCR product quantification are the two most used techniques for accurate DNA quantification. Real-time quantitative PCR can be used with different quantitative tools such as DNAbinding dyes (Morrison et al. 1998), fluorescent oligonucleotides (Whitcombe ...
enabling quantitation of the PCR product. This technique is used to detect the presence of pathogens and to determine the copy number of DNA sequences of interest.The final acronym ‘RT-qPCR’ is used for reverse transcription quantitative real-time PCR. This is a technique which combines RT-...