US6613560 2000年2月11日 2003年9月2日 Agilent Technologies, Inc. PCR microreactor for amplifying DNA using microquantities of sample fluidUS6613560 * Feb 11, 2000 Sep 2, 2003 Agilent Technologies, Inc. Polymerase chain reaction (PCR); device does not contain silicon and is thermally, chemically...
Polymerase chain reaction (PCR) is a laboratory procedure that can create replicas of DNA. Explore the three steps of this revolutionary process: denaturation, annealing, and extension. Related to this Question If a DNA replication error is detected ...
Objective: To clone and analysis light and heavy chain genes of monoclonal antibody against H1N1 influenza virus hemagglutinin by Nested PCR,then construct a common method for cloning light and heavy chain variable region genes of mouse anti-human H1N1 influenza virus hemagglutinin monoclonal antibody....
Quantification of ochratoxin A (OTA)-producing molds in foods by real-time quantitative PCR (qPCR) may be affected by the DNA extraction method used. In th... A Rodríguez,M Rodríguez,MI Luque,... - 《Food Control》 被引量: 34发表: 2012年 ...
An LM-PCR method capable of evenly amplifying DNA fragments with various GC content Kinematic and thermodynamic quantities derived from wind profiler triangles are used to help describe the structure of both an amplifying and decaying baro... S. Ishihara,N. Kotomura,Naoki Yamamoto,... - The Mol...
In order to obtain correct sequence, new primer f3x was designed using sequenced DNA, and primers f3x and p14 (or p15) were used to perform PCR and products sequencing using extracted DNA as model. Result showed that using this method, a sequence of the fanesyl pyrophosphate synthase gene...
We have constructed a novel DNA expression vector based on Semliki Forest virus (SFV). SFV produces nonstructural proteins (nsPs) which replicate genomic R... Kohno,Emi,Kasai,... - 《Gene Therapy》 被引量: 104发表: 1998年 Development of a quadriplex polymerase chain reaction for human cytome...
As the average size of the DNA in a degraded sample approaches the size of the target sequence, the amount of PCR product generated is reduced. This is due to the reduced number of intact templates in the size range ...
Amplifying heterologous, single stranded dna complementary to a gene with random oligonucleotide primers; prematurely terminating synthesis forming variabl... Disclosed is a process of performing Sexual PCR which includes generating random polynucleotides by interrupting or blocking a synthesis or amplificatio...
High speed process and apparatus for amplifying DNA Much faster thermocyclers can be constructed using pressurized gas delivered to a thermostated reaction chamber by computer-controlled electronic valves. A novel process, high-speed gas phase PCR, is described. This process has been ... A Quintana...