"A novel helper phage that improves phage display selection efficiency by preventing the amplification of phages without recombinant protein." Nucleic Acids Res 31(11): e59.Kramer RA, Cox F, van der Horst M, van der Oudenrijn S, Res PC, Bia J, Logtenberg T, de Kruif J: A novel ...
Several systems have been developed to prevent packaging of the helper viral genomes during the HD-Ad vector rescue/amplification process in order to minimize the helper virus contamination. The Cre/loxP-based system for the generation of HD-Ad involves the use of a first-generation helper virus...
Phage display technology involves the display of proteins or peptides, as coat protein fusions, on the surface of a phage or phagemid particles. Using standard technology, helper phage are essential for the replication and assembly of phagemid particles, during library production and biopanning. We ...
Amplification of IL-2-driven T cell proliferation by recombinant human IL-3 and granulocyte-macrophage colony-stimulating factor. J Immunol 1988; 141: 519–526. 45. Shannon MF, Himes SR, Coles LS. GM-CSF and IL-2 share common control mechanisms in response to costimulatory signals in T ...
“DNA Amplification of Adeno-Associated Virus as a Response to Cellular Genotoxic Stress,” Cancer Research 48:3123-3129. Primary Examiner: HILL, MYRON G Attorney, Agent or Firm: MORRISON & FOERSTER LLP (PALO ALTO, CALIFORNIA, US) Parent Case Data: CROSS-REFERENCE TO RELATED APPLICATIONS This ...
The improvement is achieved through a new mutant filamentous helper phage which has retained the gene III promoter, whereas the gene III encoding sequence is deleted. Amplification of phage titer of 10 6 times were achieved in M13-derived phages, when used for the selection of specific antibody....