Interestingly, the interference of the pirate phage to the helper phage occurs in a different manner between the SaPI-helper system and the P4-P2 system. SaPIs cannot lyse a helper lysogen upon infection, while when a helper phage lyse a SaPI lysogen, most of the phage particles produced ...
求翻译:ZAP Express vector with the aid of ExAssist helper phage (Stratagene,是什么意思?待解决 悬赏分:1 - 离问题结束还有 ZAP Express vector with the aid of ExAssist helper phage (Stratagene,问题补充:匿名 2013-05-23 12:21:38 与援助exassist辅助噬菌体(Stratagene公司ZAP表达载体, 匿名 2013...
Although the most intuitive form of phage defense involves the direct rescue of an infected cell, for example, by targeted degradation of phage nucleic acids by CRISPR-Cas or restriction modification systems, many phage-defense systems function solely at the population level. In a mechanism conceptua...
The extra domain B splice variant (EDB) of human fibronectin selectively expressed in the tumor vasculature is an attractive target for cancer imaging and therapy. Here, we describe the generation and characterization of EDB-specific optical imaging prob
Small-scale rescue was performed via VCS-M13 helper phage and the phage supernatant was used to screen via SARS-CoV-2 RBD or S1 coated ELISA plates. Positive wells were selected for colony PCR and subsequent sanger sequencing. Unique sequences were then cloned into the appropriate expression ...
recombinant MV by using a helper cell-based rescue system [111]. This approach was used to develop MERS-CoV vaccine [112], SARS-CoV vaccine [113, 114], malaria vaccine [115], and HIV-1 vaccine [116]. Currently, rMV expressing spike glycoprotein SARS- CoV-2 or nucleoprotein (N) of ...
Panel D depicts IFN-sensitive response element (ISRE) activity induction upon stimulations with IFN-α2, IFN-ω, or IFN-β in IFNAR1−/− cells where different WT or mutant IFNAR1 plasmids were transiently transfected and the H263fs* mutant does rescue the response in con- trast ...
Scryfall runs the Tagger project, which allows Scryfall supporters in the community to tag the artwork and functional pieces of each Magic card. We import these tags daily, and you can search for them on Scryfall.You can use art:, atag:, or arttag: to find things in a card’s ...
AAV vectors can have one or more of the AAV wild-type genes deleted in whole or part, preferably the rep and/or cap genes, but retain functional flanking ITR sequences. Functional ITR sequences are necessary for the rescue, replication and packaging of the AAV virion. Thus, an AAV vector...
AAV vectors can have one or more of the AAV wild-type genes deleted in whole or part, preferably the rep and/or cap genes, but retain functional flanking ITR sequences. Functional ITR sequences are necessary for the rescue, replication and packaging of the AAV virion. Thus, an AAV vector...