A novel helper phage that improves phage display selection efficiency by preventing the amplification of phages without re- combinant protein. Nucleic Acids Res 2003;31:e59.Kramer RA, Cox F, van der Horst M, van
As with the Ad/AAV hybrid system, the presence of an AAV ITR-flanked cassette in the HSV amplicon vector can promote both extrachromosomal amplification and integration of the transgene cassette into the host genome. The HSV/AAV hybrid vector system has been demonstrated to stably transform ...
Amplification of IL-2-driven T cell proliferation by recombinant human IL-3 and granulocyte-macrophage colony-stimulating factor. J Immunol 1988; 141: 519–526. 45. Shannon MF, Himes SR, Coles LS. GM-CSF and IL-2 share common control mechanisms in response to costimulatory signals in T ...
Lysate from amplifications was used to infect H16 cells at an MOI of 5. Hirt DNA was isolated. PCR was done on the Hirt DNA using primers flanking the FRT sites. The PCR products corresponding to the unflipped product were cloned into the pCR2.1-TOPO vector and sequenced. Preparation of...
et al., Amplification, overexpression, and rearrangement of the erbB-2 protooncogene in primary human stomach carcinomas. Cancer Res, 1989. 49(23): p. 6605-9. Partidos, C.D. et al., Specificity of the T-cell responses in covalently linked peptides each comprising of a T helper ...
The helper dependent vector may be linear or circular, e.g., a plasmid, depending on the particular system and protocol in which the vector is to be used. In certain embodiment, the helper dependent vector may be encapsulated in an adenoviral capsid....
et al., Amplification, overexpression, and rearrangent of the erbB-2 protooncogene in primary human stomach carcinomas. Cancer Res, 1989. 49(23): p. 6605-9. Partidos, C.D. et al., Specificity of the T-cell responses in covalently linked peptides each comprising of a T helper epitope...