A new helper phage and phagemid vector system improves viral display of antibody Fab fragments and avoids propagation of insert-less virions[J] . Glenn Soltes,Heather Barker,Kristine Marmai,Elaine Pun,Amy Yuen,Erik J. Wiersma.Journal of Immunological Methods . 2002 (1)...
Phagemid vector is converted into infective phage by superinfection of phagemid-bearing cells with helper phage, as described in the text. Reprinted with permission from Ref. 21. Advantage of phagemids Phages and phagemids are the most common vectors used in phage display, while phagemids are ...
pBC phagemids contain a 454-nucleotide filamentous f1 phage intergenic region (M13 related). The (+) and (–) orientations of the f1 intergenic region allow the rescue of sense or antisense ssDNA by a helper phage. This ssDNA can be used for dideoxynucleotide sequencing (Sanger method) or ...
of the CH1 constant region domain and the hydrophilic pig brain alpha-tubulin peptide sequence EEGEFSEAR. The vector backbone further provides a strong promotor, the T7 terminator, the ColE1 origin of replication, the intergenic region of phage F1 and an ampicillin resistance marker for selection...
(table II). After infecting cultures of vector constructs pB3, pRlac3 and pRpsp2 with VC3M13 helperphage, cells were grown overnight. The number of particles carrying phagemid DNA and helperphage DNA was compared. Surprisingly, it was observed that both new vectors show an improved packaging ...
Filamentous phage comprising a matrix of cpVIII proteins encapsulating a genome encoding first and second polypeptides of an antogenously assembling receptor, such as an antibody, a
The phagemid encoding the polypeptide-capsid protein fusion is packaged into phage particles following infection of a host bacterium with a helper phage that supplies all the structural proteins. Phage DisplaySpringer Berlin Heidelberg
The present invention provides a novel helper phage and phagemid and phagemid display system that comprises an amber mutation in gene 3 of the helper phage so that it is not expressed in the non-permissive bacteria and an in-frame stop codon in the phagemid prior to the gene 3 coding ...
(c) the progeny phage are exposed to a protease capable of cleaving the proteolytically cleavable site such that the cleavage of the protein derived from the helper phage impairs its ability to mediate infection and (d) the progeny are propagated by infection. The helper phage has the protea...
SELECTION SYSTEM FOR PHAGEMIDS USING PROTEOLYTICALLY SENSITIVE HELPER PHAGEJEAN-LUC * JESTINPETER * KRISTENSENLUTZ * RIECHMANNGREGORY PAUL * WINTER