Cryogenic Transmission Electron Microscopy (Cryo-TEM) is the state-of-the art approach for imaging and structure determination of a wide range of macromolecular assemblies at closest to their native state. Recent development in Cryo-EM hardware: automatic loading and direct electron detectors, and ...
To avoid destroying the samples, they must ensure these grids remain at cryogenic temperatures for all subsequent steps in the workflow including clipping, imaging, and storage. To store cryo-EM samples, researchers commonly place the individual vitrified grids in cryo-EM grid boxes. They...
A new cryo-EM system for single particle analysis A new cryo-EM system has been investigated for single particle analysis of protein structures. The system provides parallel illumination of a highly-cohere... T Hamaguchi,S Maki-Yonekura,H Naitow,... - 《Journal of Structural Biology》 被引量...
Corrigendum: Molecular Dynamics to Predict Cryo-EM: Capturing Transitions and Short-Lived Conformational States of Biomolecules Low resolution density maps obtained for protein flexible domains, as well as the ensemble of coexisting conformational states arising from cryo-EM, poses ... U Nierzwicki,G...
Gripper Tool for Grid Boxes 载网盒钳,铝质材质,专为低温金属载网盒或带销型盖载网盒设计 M-CEM-NS-CT01 NanoSoft Cryo Tweezers 低温镊子,适用于FEI Vitrobot各代产品,用于样品玻璃化 MD16-100 Cryo-EM grid-box storage puck 1 puck,低温载网盒储存槽 MD16-102 Cryo-EM grid-box storage puck 5 puck...
TEM Calibration Grid Holder GSB-100 Storage Boxes for FIB Lift Out Grids FIB Grid Box for Lift-Out Grids or Half Grids NEW PELCO® FIB Grid Storage PELCO® Clamping TEM Grid Holder Block Cryo Grid Boxes Cryo Grid Box Handling Tools PELCO® TEM Grid Holder BlocksPELCO...
从贝里曼和Unwin1开始,多个组已经实现了Cryo-EM在次秒时间尺度(时间解析的低温-EM)上捕获和揭示蛋白质瞬态构象状态的潜力,其技术基于杜博切特为制备低温-EM网格2而开发的标准跳动冻结方法。他们在低温杯上方添加了一个雾化剂,该雾化器使用压缩氮气将第二个样品的细雾喷洒到一个凹陷的EM网格上,其中含有第一个样品,...
In cryo-electron microscopy (cryo-EM), sample preparation poses a critical bottleneck, particularly for rare or fragile macromolecular assemblies and those suffering from denaturation and particle orientation distribution issues related to air-water interface. In this study, we develop and characterize an...
High contrast cryoEM grid storage boxes for holding clipped or unclipped grids. Designed to fit grid box sockets of the FEI Vitrobot™, the Gatan Cryo Workingstation™, FEI clipping and loading station, Leica plunger and most other current cryo-TEM devices and stations. This product is not...
We provide high quality, smooth surface molybdenum grid, (Mo grid), which is the best grid in the world for 2-D crystals study of cryo-EM.