[8]Robert G, Xiaomang Y, Baggio LL, et al. Cardiac function in mice lacking the glucagon-like peptide-1 receptor. Endocrinology, 2003, 144 : 2242–2252. DOI:10.1210/en.2003-0007
Pax6:配对盒6;CDX2/3:尾型同源盒2/3;MafB: MAF bZIP转录因子B;cMaf: c-Maf诱导蛋白;NKX2.1:NK2同源盒1;PDX1:胰腺和十二指肠同源盒1;Pax4:配对盒4;CRE:cAMP反应元件;CREB:cAMP反应元件结合蛋白;PPG:前胰高血糖素原;H...
应用领域:Cre工具鼠,Glp1r-IRES-Cre小鼠与含有loxP侧翼序列的小鼠交配时,Cre介导的重组将导致后代表达Cre细胞中floxed 序列的缺失。GLP1R 是存在于许多细胞类型上的受体,包括胰腺 β 细胞、中枢神经系统中的神经元亚群,也投射到胃肠道。 *使用本品系发表的文献需注明: Glp1r-IRES-Cre mice (Cat. NO. NM-KI-20...
Pax6:配对盒6;CDX2/3:尾型同源盒2/3;MafB: MAF bZIP转录因子B;cMaf: c-Maf诱导蛋白;NKX2.1:NK2同源盒1;PDX1:胰腺和十二指肠同源盒1;Pax4:配对盒4;CRE:cAMP反应元件;CREB:cAMP反应元件结合蛋白;PPG:前胰高糖素原;HN...
a, Representative image and quantification of viral expression (EGFP) in Glp1r-expessing neurons of Glp1r-ires-Cre mice (RNA in situ hybridization). b, Representative images of Glp1r expression (RNA in situ hybridization) in the DVC, ARC, and NG of control mice or mice with GLP1R neuron...
GLP1R: The first Glp1rCre mice were produced by inserting an iCre flanked by 5’ and 3’ Glp1r gene sequences into a murine-based bacterial artificial chromosome (BAC), followed by pronuclear injection of BAC-DNA and random integration into the genome. These mice express Cre-recombinase und...
3.2 小分子激动剂 2007 年, 人们通过高通量筛选方法从多达 48 000 个的合成和天然化合物中筛选出两个 GLP-1R 非肽激动剂,Boc5[51]和它的类似物 S4P.它们会 在 DMSO 长期储存液中自发形成二聚化合物.基于 CRE- 荧光素酶分析表明,Boc5 在 HEK-293 细胞表 达 rGLP-1R 中扮演着完全激动剂的角色并具有...
Compared with Flox/cre littermates, improved glucose tolerance, increased active GLP-1 level in pancreas and plasma, upregulated plasma insulin level in response to glucose, and decreased plasma glucagon level were observed in Fam3aα-/- mice. Primary islets isolated from Fam3aα-/- mice also...
labeled dissociated islet cells from Glp1rCre reporter mice with monoclonal antagonistic antibody, before sorting cells and quantifying promoter activity, mRNA expression and protein expression [15]. Using this approach, transcript and protein expression was abundant in beta cells, but undetectable in ...
[0070] 步骤15 =GLP-1融合蛋白质降低二型糖尿病小鼠(db/db Mice)血糖浓度的长效性研究(72 h)。 [0071] 在不同时间点测定二型糖尿病小鼠血糖浓度以评价本专利的GLP-1融合蛋白质(优选抗体GLP-1 (A8G)-LK-L13H13))在二型糖尿病小鼠体内降低其血糖浓度的长效性。两组中每组包含至少六只小鼠。在注射开始前取...