A+T content = 29.04% Nucleotide Number Mol% A 173 14.27 C 438 36.14 G 422 34.82 T 179 14.77 解决: 1.由于基因中GC含量过高,就不应该用普通的buffer了,而应该用特殊的---GC buffer,对于TM值可以做一下梯度PCR,GC buffer可以在一些公司都能买到。买TAKALA的GC buffer for PCR.还有,设计一段合适的引...
MolecularWeight=370809.00Daltons,singlestrandedMolecularWeight=741128.00Daltons,doublestrandedG+Ccontent=70.96% A+Tcontent=29.04%NucleotideNumberMol%A17314.27 C43836.14 G42234.82 T17914.77 解决: 由于基因中GC含量过高,就不应当用一般的buffer了,而应当用特别的---GCbuffer,对于TM值可以做一下梯度PCR,GCbuffer可以...
Polymerase chain reaction (PCR) is a widely utilized tool where GC content is of high importance. DNA strands must be broken apart (denatured), and primers must be able to bind to the correct position on the DNA strand (annealing) for amplification. The distribution of nucleotides can ...
1.StandardPCRamplification.HighlyGCrichregionspreventtemplatedenaturation,andhenceproductsynthesis.2.MultiplexPCRamplification.Thepreferentialamplificationoflow-GCcontentsequencesresultsinmisinterpretationofthebalancebetweenthetwosequences.7 •多重PCR•MultiplexPCR 多重PCR(MPCR)或复合PCR，它是在同一 PCR反应体系里...
to amplify using conventional PCR techniques due the presence of secondary structures that resist denaturation and prevent efficient primer annealing. Takara Bio offers several DNA polymerases designed to handle such challenging targets, with amplification of templates that contain up to 90% GC content. ...
GC content-based pan-pox universal PCR assays for poxvirus detection. J Clin Microbiol. 2010;48:268-76. http://dx.doi.org/10.1128/JCM.01697-09Li Y, Meyer H, Zhao H, Damon IK. GC content-based pan-pox universal PCR assays for poxvirus detection. J Clin Microbiol. 2010;48:268-76....
A+T content = 29.04% Nucleotide Number Mol% A 173 14.27 C 438 36.14 G 422 34.82 T 179 14.77 解决: 1.由于基因中GC含量过高,就不应该用普通的buffer了,而应该用特殊的---GC buffer,对于TM值可以做一下梯度PCR,GC buffer可以在一些公司都能买到。买TAKALA的GC buffer for PCR.还有,设计一段合适的引...
《pcr中GC含量过高的问题》由会员分享,可在线阅读,更多相关《pcr中GC含量过高的问题(4页珍藏版)》请在人人文库网上搜索。 PCR 中 GC 含量过高的问题 DNA molecule 4849 Length 1212 base pairs Molecular Weight 370809 00 Daltons single stranded Molecular Weight 741128 00 Daltons double stranded G C content ...
第三阶段:PCR扩增和Sanger测序实验验证,4周。 第四阶段:实验结果分析和总结撰写,4周。 六、参考文献 1. Li F, Liang C, Li Y, et al. High GC content of target DNA may reduce the efficiency of amplification of the dredging sediment DNA[J]. Pol J Microbiol, 2019, 68(3): 399-407. 2. ...
PCR高GC区测序确实是个挑战,因为高GC含量的区域在测序过程中不容易被测到,产生的reads较少,覆盖度也相对较低。不过,别担心,我们有一些解决方案可以帮助您更好地进行高GC区测序。比如,我们可以提供特定的GC Buffer来优化PCR扩增体系,提高高GC含量区域的扩增效率。另外,我们还可以根据您的具体需求,为您定制合适的测序...