gamma-H2AX fociprotonspulsed irradiationIntroduction: Classical particle accelerators offer proton pulses of some milliseconds duration. In contrast, the new technology of the high-intensity laser acceleration will produce ultimately shorter particle packages (up to one nanosecond) with substantially lower ...
因此,应用γH2AX染色评估DSBs损伤需保持谨慎,必须充分考虑损伤诱导因素并结合53BP1foci分析等综合评价? 文2:DNA双链断裂修复蛋白在宫颈癌和CIN组织中的表达放射线主要是通过导致DNA双链断裂(DNA double strand break,DSB)起到杀死肿瘤细胞的作用,但细胞都有不同程度的DSB修复能力,修复方式有两种:以DNA依赖蛋白激酶(...
Within minutes of DSB generation, H2AX molecules are phosphorylated in large chromatin domains flanking DNA double-strand breaks (DSBs); these domains can be observed by immunofluorescence microscopy and are termed gammaH2AX foci. H2AX phosphorylation is believed to have a role mounting an efficient ...
The number of gamma-H2AX foci per nucleus was counted by flow cytometry. The statistical differences between experimental groups at 2, 24, and 72 h were determined with a repeated measure's analysis of variance. The significant difference between groups at the same time was analyzed with the ...
07-Oct-2024 数量 批量或定制请求 添加到购物车 保存到列表 性能保障 Application Created with Sketch. 图:1/2 Phospho-gamma-H2AX (Ser139) Antibody (MA5-33062) in ICC/IF Immunocytochemistry analysis of HeLa cells, using Phospho-gamma-H2AX (Ser139) Recombinant Monoclonal antibody (Product # MA5-33...
(ATR) in the PI3K pathway. Gamma-H2AX is the first step in recruiting and localizing DNA repair proteins. DSBs can be induced by mechanisms such as ionizing radiation or cytotoxic agents and subsequently, gamma-H2AX foci quickly form. These foci represent the DSBs in a 1:1 manner and...
et al. A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Current biology: CB 10, 886–895 (2000). Article CAS PubMed Google Scholar Thiriet, C. & Hayes, J. J. Chromatin in need of a fix: phosphorylation of H2AX connects chromatin to...
The findings that LBH589 confines HDAC4 to the cytoplasm and increases the duration of gamma-H2AX foci in irradiated cell lines suggest that HDAC4 participates in DNA damage signaling following IR. Annexin-propidium iodide flow cytometry assays, cell morphology studies, and cleaved caspase-3 Western...
gamma-H2AX fociprotonspulsed irradiationIntroduction: Classical particle accelerators offer proton pulses of some milliseconds duration. In contrast, the new technology of the high-intensity laser acceleration will produce ultimately shorter particle packages (up to one nanosecond) with substantially lower ...
Introduction The detection of gamma-H2AX foci in peripheral blood mononucleated cells (PBMCs) has been incorporated as an early assay for biological dosimetry. However, overdispersion in the gamma-H2AX foci distribution is generally reported. In a previous study from our group, it was suggested ...