Elimina- tion of radiation-induced γ-H2AX foci in mammalian nucleus can occur by histone exchange. Biochem Biophys Res Commun 2007;358:650-4.Elimination of radiation-induced gamma-H2AX foci in mammalian nucleus can occur by histone exchange. Svetlova M,Solovjeva L,Nishi K,et al. Biochemical...
γH2AX foci has been generally accepted as a biomarker of DNA double-strand break(DSB).To investigate the feature and characteristics of H2AX phosphorylation(γH2AX) related to cell cycle progression,HeLa cells were synchronized at prometaphase by thymidine double-blocking followed by nocodazole treatme...
The induction and disappearance of DNA double strand breaks (DSBs) after irradiation of G1 and mitotic cells were compared with the γ-H2AX foci assay and a gel electrophoresis assay. This is to determine whether cell cycle related changes in chromatin structure might influence the γ-H2AX assay...
Phospho-H2AX fociS-phase checkpointsImmunocytochemistry using 伪-phospho-H2AX antibodies shows that hydroxyurea (HU), an inhibitor of ribonucleotide reductase, and aphidicolin (APH), an inhibitor of DNA-polymerases 伪 and 未, may promote formation of phospho-H2AX foci in late S/G2-phase cells in...
To evaluate if Interstitial γH2AX foci might be signatures of misrejoining, an mFISH analysis was performed on the same slides. The results show that Interstitial γH2AX foci lying on apparently intact chromatin do not mark sites of misrejoining, and that misrejoined events were never ...
However, foci of phosphorylated H2AX (H2AX) appear during DNA replication also in the absence of exogenously applied injury. We measured the amount and the number of foci of H2AX in different phases of the cell cycle by flow cytometry, sorting and microscopy in 4 malignant B-lymphocyte cell ...
KremplerMarlisFrankenberg-SchwagerSDOSRadiotherapy and OncologyBeucher, A.; Deckbar, D.; Schumann, E.; Krempler, A.; Frankenberg-Schwager, M.; Lobrich, M. Elevated radiation-induced gammaH2AX foci in G2 phase heterozygous BRCA2 fibroblasts. Radiother. Oncol. 2011, 101, 46-50....
In the drug-resistant derivative lines there was overall a significant increase in the number and persistence of both 纬-and foci in the nuclei of over a 72-hour period, when compared to the non-resistant parental lines (ANOVA p < 0.0001). In a Pt-resistant line (A2780cis(R)) a ...
The conventional γ-H2AX assay detects individual nuclear foci manually, but is labor-intensive and time-consuming, and hence unsuitable for high-throughput screening in cases of large-scale radiation accidents. We have developed a high-throughput γ-H2AX assay using imaging flow cytometry. This ...
目的 应用γ-H2AX分析检测电离辐射导致肝癌细胞株HepG2基因组DNA双链断裂的情况,并检测在不同细胞周期时相中的表达差异,从而了解不同时相HepG2细胞株的辐射敏感性.方法 利用胸腺嘧啶核苷(TdR)阻断HepG2细胞于G1期末,于不同时间点分别得到同步化的S期和G2/M期细胞,用60Coγ射线照射细胞,建立DNA双链断裂模型,采用...