γ-H2AX data for cell samples exposed to 1 Gy of 20 MeV protons at pulsed or continuous irradiation modes were 23.29 ± 2.04 and 26.54 ± 2.54 foci per cell, respectively. The corresponding RBE values for 20 MeV protons were 0.96 ± 0.18 and 1.13 ± 0.21 (p=0.21) for pulsed and ...
DSBs损伤形成后其断裂点的组蛋白H2AX(组蛋白H2A变体)139位丝氨酸被迅速磷酸化形成所谓的γH2AX而参与DNA损伤应答过程,用特异性抗体染色后可在荧光显微镜下看到相应的焦点(foci),γH2AXfoci分析已被公认为是DSBs损伤的标志之一[4-5]?53BP1是另外一种参与DSBs损伤应答的关键蛋白,其foci分析也成为评价DSBs损伤的标志[3...
Within minutes of DSB generation, H2AX molecules are phosphorylated in large chromatin domains flanking DNA double-strand breaks (DSBs); these domains can be observed by immunofluorescence microscopy and are termed gammaH2AX foci. H2AX phosphorylation is believed to have a role mounting an efficient ...
The manner of gamma-H2AX expression was not the same for three groups in time. The number of gamma-H2AX foci between the three groups was a significant difference after 72 h. Conclusion Simultaneous Wi-Fi and gamma-ray exposures can increase the number of double-strand break DNA in peripheral...
gamma-H2AX foci quickly form. These foci represent the DSBs in a 1:1 manner and can be used as a biomarker for damage. The visualization of gamma-H2AX by can allow the assessment of DNA damage, related DNA damage proteins and DNA repair. Gamma-H2AX has applications in the detection of ...
Therapies that either block γ-H2AX foci formation by inhibiting upstream kinase activity or that directly inhibit H2AX function may interfere with DNA damage repair processes and warrant further investigation as potential radiosensitizing agents. Agents that increase persistence of γ-H2AX after IR are ...
gamma-H2AX foci quickly form. These foci represent the DSBs in a 1:1 manner and can be used as a biomarker for damage. The visualization of gamma-H2AX by can allow the assessment of DNA damage, related DNA damage proteins and DNA repair. Gamma-H2AX has applications in the detection...
et al. A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Current biology: CB 10, 886–895 (2000). Article CAS PubMed Google Scholar Thiriet, C. & Hayes, J. J. Chromatin in need of a fix: phosphorylation of H2AX connects chromatin to...
The findings that LBH589 confines HDAC4 to the cytoplasm and increases the duration of gamma-H2AX foci in irradiated cell lines suggest that HDAC4 participates in DNA damage signaling following IR. Annexin-propidium iodide flow cytometry assays, cell morphology studies, and cleaved caspase-3 Western...
Activated cAMP signaling also delayed the disassembly of γ-H2AX foci, known as DNA damage biomarker, following gamma ray irradiation in H1299 and A549 NSLC cells. Moreover, activation of cAMP signaling delayed the repair of I-SceI endonuclease-induced DNA DSBs when the repair was measured by ...