Gamma-H2AX Foci Counting: Image processing and control software for high-content screening - Barber, Pierce, et al. - 2007Barber P, Locke R, Pierce G, Rothkamm K, Vojnovic B. γ-H2AX foci counting: image processing and control software for high- content screening. Proceedings of SPIE 2007...
After single acute (high-dose-rate) exposures, differences in mean numbers of gamma-H2AX foci per cell between samples from Atm+/+ and Atm-/- mice were clear at nearly all sampling times, but at no sampling time was there a clear distinction for cells from Atm+/+ and Atm+/- mice. ...
(2007). gamma-H2AX foci formation in peripheral blood lymphocytes of tumor patients after local radiotherapy to different sites of the body: Dependence on the dose-distribution, irradiated site and time from start of treatment. International Journal of Radiation Biology: Vol. 83, No. 10, pp. 63...
(ATR) in the PI3K pathway. Gamma-H2AX is the first step in recruiting and localizing DNA repair proteins. DSBs can be induced by mechanisms such as ionizing radiation or cytotoxic agents and subsequently, gamma-H2AX foci quickly form. These foci represent the DSBs in a 1:1 manner and...
These foci represent the DSBs in a 1:1 manner and can be used as a biomarker for damage. The visualization of gamma-H2AX by can allow the assessment of DNA damage, related DNA damage proteins and DNA repair. Gamma-H2AX has applications in the detection of ...
Quantitation of gammaH2AX foci has also been applied as a useful tool for the evaluation of the efficacy of various developmental drugs, particularly, radiation modifying compounds. This review focuses on the current status of gammaH2AX as a marker of DNA damage and repair in the context of ...
Mean Frequency and Relative Fluorescence Intensity Measurement of gamma-H2AX Foci Dose Response in PBL Exposed to gamma-Irradiation: An Inter- and Intra-Laboratory Comparison and its Relevance for Radiation Triage 机译:γ射线辐照的PBL中γ-H2AX病灶剂量响应的平均频率和相对荧光强度测量:实验室间和实验室...
It has been previously demonstrated that scoring of radiation-induced foci of the phosphorylated histone gamma-H2AX, which form at the sites of DNA double-strand breaks, may be used to determine radiation exposure levels from blood samples. Although faster than the 'gold standard' dicentric assay,...
In directly irradiated cells, the number of gamma-H2AX foci peaked 30 min after irradiation and then declined at a relatively steady pace as the cell repaired the DNA damage. Radiation effects were still detectable after 48 hrs for doses greater than 1 Gy and remained linear to initial dose....
et al. A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Current biology: CB 10, 886–895 (2000). Article CAS PubMed Google Scholar Thiriet, C. & Hayes, J. J. Chromatin in need of a fix: phosphorylation of H2AX connects chromatin to...