buccal cellsionizing radiationDNA double strand breaks are induced by ionizing radiation (IR), leading to the phosphorylation of the core histone protein H2AX (termed H2AX). The understanding of the H2AX responses in irradiated human buccal cells is still very limited. We used visual scoring and ...
下单后预计有货时间 25-Feb-2025 数量 批量或定制请求 性能保障 说明书 实验方案 技术支持 Application Created with Sketch. 图:1/6 gamma-H2AX Antibody (A700-053) in ICC/IF Detection of human gamma-H2AX by immunocytochemistry. Sample: FFPE section of human HeLa cells treated with etoposide. Antibody...
Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. 靶标信息 H2AX is a variant of the H2A protein family and is a component of the histone octomer in nucleosomes. H2AX...
Effect and Mechanism of Radiosensitization of Poly (ADP-Ribose) Polymerase Inhibitor on Lewis Cells and Xenografts Background and objective The DNA damage of the irradiated tumor cells is mainly single strand breaks(SSBs) and double strand breaks(DSBs), in which the fre......
Cancer develops due to the accumulation of genetic and epigenetic changes caused by various factors. An important source of genetic changes is DNA double-strand breaks (DBSs). Radiation, oxidative stress, and carcinogens in the environment cause DNA DSBs. Cells respond to DSBs by phosphorylating his...
These conclusions derive from immunocytological analysis of a phosphorylated histone variant. gamma -H2AX, previously found to be characteristic of DSB repair in mitotic cells, and shown here to be recruited for specialized use during meiosis. 展开 关键词:...
et al. Wip1 regulates the generation of new neural cells in the adult olfactory bulb through p53-dependent cell cycle control. Stem cells 27, 1433–1442 (2009). Article CAS PubMed Google Scholar Fernandez, F. et al. Wip1 phosphatase positively modulates dendritic spine morphology and memory...
Immunocytochemistry of HeLa cells using gamma H2AX Rabbit mAb RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green). 产品留言 标题 联系人 联系电话 内容 验证码 点击换一张 注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
Cells exposed to non-thermal environmental conditions that lead to protection from, or sensitization to, heat were also tested. The heat sensitivity of cells in which H2AX was knocked out was also ascertained. Results: The protein synthesis independent state of thermotolerance, but not the protein ...
2.4. Peripheral blood mononuclear cells isolation Using gradient centrifugation method, the peripheral blood mononuclear cells (PBMCs) were isolated as follows at Room Temperature (RT). Two ml of blood samples transferred into a heparinized tube and 2 cc of PBS was added to it in Falcon tubes ...