Age- and oxidative stress-induced DNA damage in Drosophila intestinal stem cells as marked by Gamma- H2AX. Exp. Gerontol. 47 (5), 401.Park JS, Lee SH, Na HJ, Pyo JH, Kim YS, Yoo MA. 2012. Age- and oxidative str
et al. Wip1 regulates the generation of new neural cells in the adult olfactory bulb through p53-dependent cell cycle control. Stem cells 27, 1433–1442 (2009). Article CAS PubMed Google Scholar Fernandez, F. et al. Wip1 phosphatase positively modulates dendritic spine morphology and memory...
HEK293 cells 分类 Recombinant Monoclonal 类型 Antibody 克隆号 BLR053F 抗原 Peptide surrounding phosphorylated serine 140 of human histone H2AX. 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 100 µg/mL 纯化类型 purified 保存液 BBS, pH 7.8 to 8.6, with 0.1% B...
Cells respond to DSBs by phosphorylating histone H2AX to produce γH2AX. In in vitro assays, analysis of γH2AX formation enables detection of genotoxic carcinogens with a high accuracy. In in vivo assays, both genotoxic and non-genotoxic carcinogens can be detected. γH2AX is highly expressed ...
2.4. Peripheral blood mononuclear cells isolation Using gradient centrifugation method, the peripheral blood mononuclear cells (PBMCs) were isolated as follows at Room Temperature (RT). Two ml of blood samples transferred into a heparinized tube and 2 cc of PBS was added to it in Falcon tubes ...
After single acute (high-dose-rate) exposures, differences in mean numbers of gamma-H2AX foci per cell between samples from Atm+/+ and Atm-/- mice were clear at nearly all sampling times, but at no sampling time was there a clear distinction for cells from Atm+/+ and Atm+/- mice. ...
Application Created with Sketch. 图:1/5 Phospho-gamma-H2AX (Ser139) Antibody (A300-081A) in ICC/IF Detection of human gamma-H2AX by immunocytochemistry. Samples: Neocarzinostatin treated asynchronous HeLa cells (left) and untreated asynchronous HeLa cells (right). Antibody: Affinity purifie...
Cells exposed to non-thermal environmental conditions that lead to protection from, or sensitization to, heat were also tested. The heat sensitivity of cells in which H2AX was knocked out was also ascertained. Results: The protein synthesis independent state of thermotolerance, but not the protein ...
Application Note: For ICC, formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. 靶标信息 ...
A novel treatment for cancer patients with homozygous deletions of BRCA1 and BRCA2 is to use drugs that inhibit the enzyme poly(ADP-ribose) polymerase (PARP). Specific inhibition of PARP-1 can induce synthetic lethality in irradiated cancer cells while t