gammaH2AX foci as a measure of DNA damage: a computational approach to automatic analysis.The gammaH2AX focus assay represents a fast and sensitive approach for the detection of one of the critical types of DNA damage - double-strand breaks (DSB) induced by various cytotoxic agents including ...
et al. A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Current biology: CB 10, 886–895 (2000). Article CAS PubMed Google Scholar Thiriet, C. & Hayes, J. J. Chromatin in need of a fix: phosphorylation of H2AX connects chromatin to...
(ATR) in the PI3K pathway. Gamma-H2AX is the first step in recruiting and localizing DNA repair proteins. DSBs can be induced by mechanisms such as ionizing radiation or cytotoxic agents and subsequently, gamma-H2AX foci quickly form. These foci represent the DSBs in a 1:1 manner and...
gamma H2AX fociDNA repairRadiation sensitivityGlioblastomaCancer stem cellsGlioma stem cellsAim: To assess radiation response using gamma H2AX assay in surgical specimens from glioblastoma (GB) patients and their corresponding primary gliosphere culture. To test the hypothesis that gliospheres (stem cell...
Introduction The detection of gamma-H2AX foci in peripheral blood mononucleated cells (PBMCs) has been incorporated as an early assay for biological dosimetry. However, overdispersion in the gamma-H2AX foci distribution is generally reported. In a previous study from our group, it was suggested ...
gamma-H2AX assayradiation-induced fociPurpose: Within the EU RENEB project, seven laboraInternational Journal of Radiation Biology: Covering the Physical, Chemical, Biological, and Medical Effects of Ionizing and Non-ionizing Radiationsdoi:10.1080/09553002.2016.1207822...
gamma H2AX assay in ex vivo irradiated tumour specimens: A novel Method to determine tumour radiation sensitivity in patient-derived materialgamma H2AX fociRadiotherapyDNA repairTumour biopsyBiomarkerPurpose: To establish a clinically applicable protocol for quantification of residual gamma H2AX foci in ex...
?-H2AX foci were enumerated in blood lymphocytes and normal fibroblasts of human and porcine origin after ex vivo ?-ray irradiation. DNA double-strand break repair kinetics in minipig blood lymphocytes and fibroblasts, based on the ?-H2AX assay, were similar to those observed in their human ...
DSB was measured by pulsed-field electrophoresis (PFGE) as well as gamma-H2AX foci formation and removal. Apoptosis induction was tested by DAPI staining and sub-G1 flow cytometry, survival was quantified by clonogenic assay./p pResults/p pTBB treatment did not affect initial DNA fragmention ...
(ATR) in the PI3K pathway. Gamma-H2AX is the first step in recruiting and localizing DNA repair proteins. DSBs can be induced by mechanisms such as ionizing radiation or cytotoxic agents and subsequently, gamma-H2AX foci quickly form. These foci represent the DSBs in a 1:1 manner and can ...