Wenwen Lv,Jie Ding,Jiangbo Hu,Chao Wang,Leyi Xu,Ming Fang,Liudi Yuan. A GAL4/UAS luciferase system to identify the miRNAs target mRNAs in Drosophila S2 cells[J]. Molecular Biology Reports . 2009 (6)Wenwen Lv,Jie Ding,Jiangbo Hu,Chao Wang,Leyi Xu,Ming Fang,Liudi Yuan.A GAL4/...
luciferase.Immtmopre· cipitation We s ter n blot was used to analyze t h e A B secretion .The expression of the reporter gene in the ce l l ly~ te s WaS assayed by the method based on GaI 4/ VP16 一UAS and Dual —L^lc】 涨reporter gene system. R esults:Lucifc“ I se ...
The development of the bipartite Gal4-UAS system in Anopheles gambiae would improve the functional characterization of genes in this important malaria vector. Towards this aim, we used Gal4 driver plasmids to successfully activate expression of the reporter gene, luciferase, from UAS responder ...
The predicted miR-275 target gene- sarco/endoplasmic reticulum Ca2+-ATPase (SERCA)-was confirmed to directly bind miR-275 by means of luciferase assay. RNA interference silencing of SERCA or application of SERCA-specific inhibitor Thapsigargin (TG) resulted in an apparent lack of blood digestion,...