We offer the Flp-In T-REx system that contains pFRT/lacZeo and pcDNA6/TR stably integrated into HEK 293 cells. This cell line has been functionally tested for its ability to regulate expression. In theory, one can get multiple integrations of the Flp-In expression construct—an FRT-specific...
Flp-In™-CV-1、Flp-In™-293、Flp-In™-CHO 和 Flp-In™ Jurkat 细胞系(图1)与表达 CMV 启动子基因的 Flp-In™ 载体(pcDNA™5/FRT、pcDNA5/FRT/V5-His-TOPO™ 和 pSecTag/FRT/V5-His-TOPO™)配合使用。Flp-In™-BHK 和 Flp-In™-3T3 细胞往往会下调 CMV 启动子。因此,建议将...
Overexpression of human LF in HEK293 cells using Flp-In T-Rex system.Byungtak, KimSeongeun, KangSun, Jung Kim
11. Chen, C., and Okayama, H. (1987). High-Efficiency Transformation of Mammalian Cells by Plasmid DNA. Mol. Cell. Biol. 7, 2745-2752. 12. Chu, G., Hayakawa, H., and Berg, P. (1987). Electroporation for the Efficient Transfection of Mammalian Cells with DNA. Nuc. Acids Res. 15...
The principle and an application example of the Double-Flp-In technique. (a) The integration of two simultaneously transfected plasmids encoding two genes of interest can be achieved into a single Flp recombination target (FRT) site. (b) As a proof of concept, we transfected HEK293 cells with...
The principle and an application example of the Double-Flp-In technique. (a) The integration of two simultaneously transfected plasmids encoding two genes of interest can be achieved into a single Flp recombination target (FRT) site. (b) As a proof of concept, we transfected HEK293 cells with...
A new generation of retroviral producer cells: predictable and stable virus production by Flp mediated site-specific integration of retroviral vectors. Mol. Ther. (in press).Schucht, R., Coroadinha, A. S., Zanta-Boussif, M. A., Verhoeyen, E., Carrondo, M. J., Hauser, H. & Wirth...
no confirmed exceptions from predicted reactivity known in the moment additional information This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7...
All receptors were expressed heterologously in HEK293 cells and similar expression levels of human andC. elegansreceptors as well as their presence in the plasma membrane was confirmed (Fig.2a). One exception wasnpr-3, with weaker expression and only a fraction of the receptor being exported to...
Wu H, Mao C, Duenstl G, Su W, Qian S (2013) Assay development of inducible human renal phosphate transporter Npt2A (SLC34A1) in Flp-In-Trex-HEK293 cells. Eur J Pharmacol 721:332–340Wu, H, Mao, C, Duenstl, G, Su, W, Qian, S (2013) Assay development of inducible human ...