Jump-In™ T-REx™ HEK293 细胞(2 小瓶,每瓶 1 mL),储存于液氮中 pJTI™ R4 DEST CMV TO pA 载体 (100 µg),储存在 -20°C 下 pJTI™ R4 Int 载体 (100 µg),储存于 -20°C 下 常见问题解答 (FAQ) The Flp-In T-REx system combines the targeted integration offered by the Fl...
Use irreversible integration (Jump-In system) if the transgene should be sustained in the mammalian genome for a long time. Use reversible integration such as Flp-In system if the transgene needs to be replaced with another gene of interest after a short period of time. ...
Overexpression of human LF in HEK293 cells using Flp-In T-Rex system.Byungtak, KimSeongeun, KangSun, Jung Kim
Flp-In 293 T-REx细胞系是为了快速生成稳定的细胞系而设计的,它可以确保从Flp-In表达载体上获得的蛋白的均匀表达。这些细胞在转录活跃的基因组位点上包含一个稳定整合的FRT位点,有针对性的整合Flp-In表达载体,确保高水平目的基因。Flp-In t -293细胞系包含稳定整合的pFRT lacZeo和pcDNA 6 TR(来自T-REx系统)。
Analysis of pre-rRNAs intermediates in HEK293 Flp-In T-Rex cells expressing a shRNA targeting the FTSJ3 mRNA.Luis, G. MorelloPatricia, P. ColtriAlexandre, J. C. QuaresmaFernando, M. SimabucoTereza, C. L. SilvaGuramrit, SinghJeffrey, A. Nickerson...
HEK293 and Flp-In-HEK293 cell lines stably transfected with hNpt2A could be selected, but these cells were inactive in phosphate transport. This suggests that high-level, constitutive Npt2A expression has deleterious effects on the cell. By using the conditional promoter in the Flp-In-Trex ...
For live-cell imaging, cells were seeded and imaged in FluoroDish cell culture dishes (World Precision Instru- ments, Hertfordshire, UK; FD-35). To enhance the adherence of the Flp-In T-REx 293 cells to the glass surface, the dishes were precoated with polyethyleneimine (PEI) (MP ...
The Flp-In T-REx system combines the targeted integration offered by the Flp-In system with the powerful inducible expression offered by the T-REx system. It allows generation of isogenic, inducible, stable cell lines and permits polyclonal selection of these cell lines. Once the Flp-In T-REx...