Peripheral blood mononuclear cells (PBMCs) were isolated, and flow cytometry was used to determine the phenotypic distribution of CD8 + T cell subsets. Expression of cytokines such as TNF-α and IFN-γ was detected by quantitative reverse transcription–PCR, a fluorescence flow cytometry-based ...
Unlocking CD8+ T cell potential in chronic hepatitis B virus infection Valeria Fumagalli Matteo Iannacone Nature Reviews Gastroenterology & Hepatology(2025) Allogeneic CD33-directed CAR-NKT cells for the treatment of bone marrow-resident myeloid malignancies ...
g, Counts of various immune cell subsets in B16F10 tumors. For endogenous CD8+ T cells, PMEL CD8+ T cells, and total CD4+ T cells, shown are pooled data of two independent experiments (n = 10 independent animals). For other immune cells, data are representative of three independent...
Prognostic significance of pleural effusion exhausted CD8+ T cell subsets in non-small cell lung cancer and mesothelioma. e21111#Background:Following repeated tumor antigen stimulation, T cells lose functional capacity and become exhausted. T cell exhaustion is a progressive c... L Ye,I Dick,P ...
More information:Jean-Christophe Beltra et al. Developmental Relationships of Four Exhausted CD8+ T Cell Subsets Reveals Underlying Transcriptional and Epigenetic Landscape Control Mechanisms,Immunity(2020).DOI: 10.1016/j.immuni.2020.04.014
Persistent infections result in dysfunctional state of CD8 T cells associated with the expression of multiple inhibitory receptors. Recent studies have attempted to elucidate T cell subsets in chronic infections. Similarly, the chemokine receptor CX3CR1 has been recently three effector and memory subsets...
CD8+ T cell exhaustion is a state of dysfunction acquired in chronic viral infection and cancer, characterized by the formation of Slamf6+ progenitor exhausted and Tim-3+ terminally exhausted subpopulations through unknown mechanisms. Here we establi
During acute LCMV-Arm infection, EGR2 expression initially spiked above naive CD8+ T cell levels at day 5 post-infection (p.i.) before dropping below naive levels after viral clearance from day 8 p.i. onwards (Fig. 1a). In contrast, during chronic LCMV-Cl13 infection, EGR2 was ...
During chronic infection, pathogen-specific CD8(+) T cells upregulate expression of molecules such as the inhibitory surface receptor PD-1, have diminished cytokine production and are thought to undergo terminal differentiation into exhausted cells. Here we found that T cells with memory-like propertie...
CD8+ T cell exhaustion is a state of dysfunction acquired in chronic viral infection and cancer, characterized by the formation of Slamf6+ progenitor exhausted and Tim-3+ terminally exhausted subpopulations through unknown mechanisms. Here we establish t