ERCC spike in controls – looking for a friend!Sophie Shaw
ERCC Bioscope Readme: ERCC RNA Spike-In Control Mixes (English ) ERCC Controls Analysis: ERCC RNA Spike-In Control Mixes (English ) User Guide: ERCC RNA Spike-In Control Mixes (English ) User Bulletin: ERCC_Analysis Plugin ERCC Controls Annotation: ERCC RNA Spike-In Control Mixes (English ...
ERCC校准方法基于一种称为“spike-in”的技术,即将已知浓度的外源性RNA分子添加到待测样本中。这些外源性RNA分子与待测样本中的内源性RNA分子一同被转录成cDNA,并通过测序技术进行测序。通过比较外源性RNA分子的实际浓度和预期浓度,可以评估RNA测序的准确性和灵敏度。 ERCC校准方法的应用非常广泛。首先,它可以用于评估...
ERCC metrology products includeStandard Reference Material (SRM) 2374for use in the production of RNA spike-in control mixtures, documentary standard CLSI MM16-A, and the erccdashboard R package, an innovative spike-in control analysis software tool. The scientific community has embraced the use o...
Whether measuring dynamic range or gene expression fold-change, Ambion™ ERCC Spike-In Control Mixes are available in two kit configurations to meet your experimental needs. Use the ERCC Spike-In Mix to determine the dynamic range and lower limit of detection on your platform, and use the ...
ERCC的RNA spike-in的结果一般是怎么分析的?我们是否有建立相应分析方法? 分析需要先从官网下载ERCC_Control s_Annotation.tx文件,该文件包含每个序列ID,组别,mix中的浓度。 得到测序序列,先进行比对,然后normalized和过滤得到表达数据(RPKM.FPKM),汇出4问中的结果图。
Includes: Control Template(s) Control Type: ERCC RNA Control Product Line: Ambion™ 储存 This kit contains:• 10 µl ERCC RNA Spike-In Mix 1• 1.75ml Nuclease-free Water-Do not store the Spike-In Mixes in a frost-free freezer.-Store ERCC RNA Spike-In Mix 1 at –20°C...
ERCC spike-in mixes QAQC.Neetu JagyaSatya Pavan Kumar VarmaDeepshi ThakralPrashant JoshiHemlata DurgapalSubrat Kumar Panda
ABSTRACT:ObjectiveToinvestigatethechangesintheexpressionofmiR一192,miR-215and ERCC3afterradiotherapyandtheirsignificanceinnon-smallcelllungcancer(NSCLC)A549cells. Methods CulturedA549cellsweredividedintothreegroups:controlgroup,20Gytreatmentgroup and40Gytreatmentgroup.TheexpressionofmiR一192andmiR一215wasmeasuredby...
无论测定动态范围或基因表达倍数变化,ERCC 内参对照混合物有两种试剂盒配置可满足您的实验需求。使用ERCC Spike-In混合物确定您在用平台的动态检测范围和检测低限,并使用ERCC ExFold Spike-In混合物评估基因表达差异测定结果的精确性。 ERCC RNA 内参混合物1*ExFold 内参混合物1*ExFold 内参混合物2*Nuclease-free水...