也正是因为这点,美国标准技术研究所NIST才决定领导开发一个RNA spike-in工具,来解决这个问题。 ERCC的RNA spike-in有哪些序列,一般是怎使用的? 设计的RNA spike-in序列长度范围是250-2000nt,GC含量在5-51%,这个长度模拟了真核生物转录本的长度状态,ERCCC 2.0版本的RNA spike-in 有96条DNA分子,每条序列都有特...
ERCC spike-in mixes QAQC.Neetu JagyaSatya Pavan Kumar VarmaDeepshi ThakralPrashant JoshiHemlata DurgapalSubrat Kumar Panda
Ambion™ ERCC Spike-In Control Mixes are available in two kit configurations to meet your experimental needs. Use the ERCC Spike-In Mix to determine the dynamic range and lower limit of detection on your platform, and use the ERCC ExFold Spike-In Mixes to assess the accuracy of differential...
而且,两种样品中表达水平幅度变化的比率,可通过ExFold RNA Spike-In 1和ExFold RNA Spike-In 2(图3)之间高度一致的关系来计算,具有很高的可信度。由于内参混合物中各种转录本的摩尔浓度已知,使用92种转录本的4种亚群的不同比值的组合对两种混合物进行关联,即可确定测量值。这些对照品是下一代测序实验(如 SOLiD...
We evaluate the performance of the External RNA Control Consortium (ERCC) spike-in controls and investigate the possibility of using them directly for normalization. We show that the spike-ins are not reliable enough to be used in ... D Risso,J Ngai,TP Speed,... - 《Nature Biotechnology》...
External RNA Controls Consortium (ERCC) ERCC是啥? 外部RNA控制联盟,就是一套RNA-seq,基因表达检测过程中的控制系统,使得结果具有可重复性。 RNA Spike-in Controls for Gene Expression While early gene expression measurements with DNA microarrays were groundbreaking in their ability to reveal biological ...
ERCC spike in controls – looking for a friend!Sophie Shaw
in order to measure against defined performance criteria. Up until the design of such universally accepted controls, it has been difficult to execute a thorough investigation of fundamental analytical performance metrics. From the trusted brand of quality RNA reagents, Ambion® ERCC Spike-In Control...
► Nucleotide excision repair is impaired in HepG2.2.15 cells. ► Altered miRNA expression including upregulation of miR-192 was observed in HepG2.2.15 cells. ► ERCC3 and ERCC4 are targets of miR-192. ► MiR-192 inhibits nucleotide excision repair. ...
无论测定动态范围或基因表达倍数变化,ERCC 内参对照混合物有两种试剂盒配置可满足您的实验需求。使用ERCC Spike-In混合物确定您在用平台的动态检测范围和检测低限,并使用ERCC ExFold Spike-In混合物评估基因表达差异测定结果的精确性。 ERCC RNA 内参混合物1*ExFold 内参混合物1*ExFold 内参混合物2*Nuclease-free水...