也正是因为这点,美国标准技术研究所NIST才决定领导开发一个RNA spike-in工具,来解决这个问题。 ERCC的RNA spike-in有哪些序列,一般是怎使用的? 设计的RNA spike-in序列长度范围是250-2000nt,GC含量在5-51%,这个长度模拟了真核生物转录本的长度状态,ERCCC 2.0版本的RNA spike-in 有96条DNA分子,每条序列都有特...
ERCC spike-in mixes QAQC.Neetu JagyaSatya Pavan Kumar VarmaDeepshi ThakralPrashant JoshiHemlata DurgapalSubrat Kumar Panda
ERCC校准方法基于一种称为“spike-in”的技术,即将已知浓度的外源性RNA分子添加到待测样本中。这些外源性RNA分子与待测样本中的内源性RNA分子一同被转录成cDNA,并通过测序技术进行测序。通过比较外源性RNA分子的实际浓度和预期浓度,可以评估RNA测序的准确性和灵敏度。 ERCC校准方法的应用非常广泛。首先,它可以用于评估...
ERCC RNA Spike-In Mix (Cat. No. 4456740) 10 μL — — 1.75 mL ERCC ExFold RNA Spike-In Mixes (Cat. No. 4456739) — 10 μL 10 μL 1.75 mL* Although ERCC RNA Spike-In Mix 1 and ExFold Spike-In Mix 1 contain the same formulation of ERCC transcripts, do not substitute ERCC RN...
而且,两种样品中表达水平幅度变化的比率,可通过ExFold RNA Spike-In 1和ExFold RNA Spike-In 2(图3)之间高度一致的关系来计算,具有很高的可信度。由于内参混合物中各种转录本的摩尔浓度已知,使用92种转录本的4种亚群的不同比值的组合对两种混合物进行关联,即可确定测量值。这些对照品是下一代测序实验(如 SOLiD...
ERCC spike in controls – looking for a friend!Sophie Shaw
The ERCC spike-in mix contains 92 transcripts of known concentration. The set is organized into four subgroups, each containing 23 controls that span six logs of dynamic range in concentration. The ERCC mix is added t...
External RNA Controls Consortium (ERCC) ERCC是啥? 外部RNA控制联盟,就是一套RNA-seq,基因表达检测过程中的控制系统,使得结果具有可重复性。 RNA Spike-in Controls for Gene Expression While early gene expression measurements with DNA microarrays were groundbreaking in their ability to reveal biological ...
Ambion® ERCC RNA Spike-In Controls are used to create a standard baseline measurement of RNA both within an experiment and across multiple experiments performed using various samples and platforms. With two spike-in mix formulations (Figure 1), various measurements, such as sensitivity or dynamic...
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