而且,两种样品中表达水平幅度变化的比率,可通过ExFold RNA Spike-In 1和ExFold RNA Spike-In 2(图3)之间高度一致的关系来计算,具有很高的可信度。由于内参混合物中各种转录本的摩尔浓度已知,使用92种转录本的4种亚群的不同比值的组合对两种混合物进行关联,即可确定测量值。这些对照品是下一代测序实验(如 SOLiD...
ERCC校准方法基于一种称为“spike-in”的技术,即将已知浓度的外源性RNA分子添加到待测样本中。这些外源性RNA分子与待测样本中的内源性RNA分子一同被转录成cDNA,并通过测序技术进行测序。通过比较外源性RNA分子的实际浓度和预期浓度,可以评估RNA测序的准确性和灵敏度。 ERCC校准方法的应用非常广泛。首先,它可以用于评估...
ERCC metrology products includeStandard Reference Material (SRM) 2374for use in the production of RNA spike-in control mixtures, documentary standard CLSI MM16-A, and the erccdashboard R package, an innovative spike-in control analysis software tool. The scientific community has embraced the use o...
无论测定动态范围或基因表达倍数变化,ERCC 内参对照混合物有两种试剂盒配置可满足您的实验需求。使用ERCC Spike-In混合物确定您在用平台的动态检测范围和检测低限,并使用ERCC ExFold Spike-In混合物评估基因表达差异测定结果的精确性。 ERCC RNA 内参混合物1*ExFold 内参混合物1*ExFold 内参混合物2*Nuclease-free水...
分析需要先从官网下载ERCC_Control s_Annotation.tx文件,该文件包含每个序列ID,组别,mix中的浓度。 得到测序序列,先进行比对,然后normalized和过滤得到表达数据(RPKM.FPKM),汇出4问中的结果图。 在不同样本之间,最后通过ERCC RNA spike-in reads作为factor,进行样本之间的矫正: ...
Control Type: ERCC RNA Control Product Line: Ambion™ 储存 This kit contains:• 10 µl ERCC RNA Spike-In Mix 1• 1.75ml Nuclease-free Water-Do not store the Spike-In Mixes in a frost-free freezer.-Store ERCC RNA Spike-In Mix 1 at –20°C-Store Nuclease-free Water at an...
Nouspikel T.DNA repair in mammalian cells:Nucleotide excision repai~ vari ations on versatility [J ]. Cell Mol Life Sci ,2Oo9 ,66( 6) :994- 1009. [ 5] Z urita M,Meri no C.The transcri ptional complexity of the TFIIH complex[J ].Trends Genet ,2003 ,19(10): ...
Nucleotide excision repair in differentiated cells Mutat. Res. (2007) T. Nouspikel DNA repair in mammalian cells: nucleotide excision repair: variations on versatility Cell Mol. Life Sci. (2009)View more references Cited by (37) MicroRNA-140 impedes DNA repair by targeting FEN1 and enhances ...
Nucleotide excision repair in differentiated cells Mutat. Res. (2007) T. Nouspikel DNA repair in mammalian cells: nucleotide excision repair: variations on versatility Cell Mol. Life Sci. (2009)View more references Cited by (37) MicroRNA-140 impedes DNA repair by targeting FEN1 and enhances ...
Control TypeRNA Control For Use With (Application)Next-Generation Sequencing IncludesControl Template(s) Product LineAmbion™ Product TypeERCC RNA Spike-In Mix Quantity1 Kit Unit SizeEach 內容物與存放 This kit contains:• 10 μl ERCC RNA Spike-In Mix 1• 1.75ml Nuclease-free Water-Do not...