而且,两种样品中表达水平幅度变化的比率,可通过ExFold RNA Spike-In 1和ExFold RNA Spike-In 2(图3)之间高度一致的关系来计算,具有很高的可信度。由于内参混合物中各种转录本的摩尔浓度已知,使用92种转录本的4种亚群的不同比值的组合对两种混合物进行关联,即可确定测量值。这些对照品是下一代测序实验(如 SOLiD...
Scientists have developed two powerful tools for RNA sequencing (RNA-Seq) experiments to minimize bias and variability: unique molecular identifiers (UMIs) and ERCC RNA spike-ins. We’ll cover the basics of each and ho...
Power analysis of single cell RNA-seq experiments using ERCC spike-insdoi:10.7490/f1000research.1113885.1Valentine SvenssonS. TeichmannF1000Research
It can be found in proteins such as DNA and RNA polymerases, DNA ligases, DNA glycosylases and in the NER repair nuclease UvrC [156,182]. Two copies of this HhH motif are connected by an α-helix (helix γ) and together this (HhH)2 motif forms a globular fold. The key residues ...