Ambion™ ERCC RNA 内参对照用于在使用多种样品和平台进行的一项实验中和多项验之间创建 RNA 的标准基线测定值。采用两种内参混合物配方(图1),可以对多种指标(如灵敏度或动态范围)进行检查,以评估一项实验中或不同实验间的不同参数(图2)。而且,两种样品中表达水平幅度变化的比率,可通过ExFold RNA Spike-In 1...
Scientists have developed two powerful tools for RNA sequencing (RNA-Seq) experiments to minimize bias and variability: unique molecular identifiers (UMIs) and ERCC RNA spike-ins. We’ll cover the basics of each and ho...
Power analysis of single cell RNA-seq experiments using ERCC spike-insdoi:10.7490/f1000research.1113885.1Valentine SvenssonS. TeichmannF1000Research
On the other hand, in TC-NER RNA Polymerase II that is stalled at a DNA lesion, triggers recognition allowing to preferentially repair the transcribed strand of genes (Figure 1). When stalled at the lesion, the Cockayne syndrome proteins CSB and CSA translocate RNA Pol II, so that the ...