NMR spectroscopy can provide information on many different aspects of proteinligand interactions-not only structural but also dynamic, kinetic and thermodynamic (Jardetzky and Roberts, 1981; Feeney and Birdsall, 1993). For protein-ligand complexes of relatively modest size (<30kD), the use of ...
The reconstituted fluorescent protein can also increase the false-positive rate. Thus, in addition to split-LUC, dynamic methods, such as BRET and FRET, should be used to decrease the false-positive rate. Biophysical analysis of interactions The formation of a dynamic multi-protein complex is ...
37-66. Chapman & Hall, London (1986).RYAN, M. D.; BELSHAM, G. J.; KING, A. M.: ' Specificity of enzyme-substrate interactions in foot-and-mouth disease virus polyprotein processing ' VIROLOGY vol. 773, no. 1, 1989, pages 35 - 45...
S Jones, J.M Thornton Principles of protein-protein interactions Proc. Natl. Acad. Sci. USA, 93 (1996), pp. 13-20 Google Scholar Jones et al. 1991 T.A Jones, J.Y Zou, S.W Cowan, M Kjeldgaard Improved methods for building protein models in electron density maps and the location of...
Probing Steric and Hydrophobic Effects on Enzyme-Substrate Interactions by Protein Engineering Steric and hydrophobic effects on substrate specificity were probed by protein engineering of subtilisin. Subtilisin has broad peptidase specificity and contains a large hydrophobic substrate binding cleft. A cons.....
This will facilitate the generation of variants with improved activity or altered selectivity in the future by means of protein engineering. 展开 关键词: CYP154C5 STEROID HYDROXYLATION NOCARDIA FARCINICA DOI: 10.1107/S1399004714019129 被引量: 20 ...
Expanding the number of 'druggable' targets: non-enzymes and protein-protein interactions. Following sequencing and assembly of the human genome, the preferred methods for identification of new drug targets have changed dramatically. Modern tacti... LN Makley,JE Gestwicki - 《Chemical Biology & Dru...
an E3 ligase, ubiquitinate the substrate. Structural insights into the process of substrate ubiquitination are limited to the transfer of one ubiquitin2or ubiquitin-like protein3,4. How specificity is achieved within the ubiquitination system remains largely elusive due to its extremely high level of...
Thousands of genomes are publicly available, however, most genes in those genomes have poorly defined functions. This is partly due to a gap between previously published, experimentally characterized protein activities and activities deposited in databases. This activity deposition is bottlenecked by the...
γ-Secretase is an aspartyl intramembrane protease that cleaves the amyloid precursor protein (APP) involved in Alzheimer’s disease pathology and other transmembrane proteins. Substrate-bound structures reveal a stable hybrid β-sheet immediately following the substrate scissile bond consisting of β1 and...