Decolourization of the 610-nm chromophore of ceruloplasmin by ascorbate is due to a ratedetermining second-order interaction between enzyme and substrate. Apparent rate constants for this process appear to be independent of the oxidation state of the 340-nm chromophore of the protein, and decrease...
The interface may be transient or long-lasting, and lead to a local functional outcome or a long-range effect. For example, many enzyme–substrate interactions may be characterized as a localized event where the structure of the active site is significant, as well as the kinetics of the ...
pU**sy 上传280.78 KB 文件格式 zip active_site:活性位点 denatured:变性的 enzyme:酶 substrate:底物 一个酶-底物相互作用模型,以帮助可视化。还包括变性酶模型。底物模型分为两部分,包括接受6毫米×3毫米钕磁铁的孔,以便方便组装。不错的3d模型库素材,仅展示其中一张素材,下载可以查看完整原格式文件素材...
This may be attributed to enzyme deactivation via a variety of possible mechanisms such as thermal, mechanical, and/or chemical activity. We have sought to determine whether the main source of enzyme deactivation is from enzyme-substrate interactions or other interactions of the enzyme in solution ...
The simulations were carried out with the AMBER 14 simulation package and repeated twice for each system. The standard AMBER force field ff99SB and GLYCAM_06j-1 were used to generate the topologies and parameters of the enzyme and substrate, respectively42,43. Each system was immersed in a do...
The use of such protein chip and methodology allows an increase in the reactivity between a peptide with low molecular weight and an enzyme with high molecular weight and between the peptide and a reactive antibody on the protein chip, so that the interaction between the peptide and the protein...
enzyme and substrate, are key determinants of their association. Yet, structure contributions are not trivial in the case of RNP associations because the combination of different nucleotides bears an obvious specificity-determining potential. While unfolded regions promote protein–protein assembly and disor...
This chapter reviewed fundamental aspects related to cluster–substrate inter- actions, and they have been illustrated with current examples from the literature. Differences between physisorption and chemisorption processes are essential to understand these inter- actions because they will determine the ...
Relative intensities of backbone amides of cytb5in a 1:1 molar ratio with CYP2B4, represented as a percentage of the corresponding resonance intensity in free cytb5, are plotted for each cytb5residue for (A) the complex between cytb5and substrate-free CYP2B4 and (D) the complex between ...
and the functional coupling of MiCK with adenine nucleotide translocase is intact in mitochondria isolated with the use of trypsin (Saks et al., 1975; =... WE Jacobus,VA Saks - 《Archives of Biochemistry & Biophysics》 被引量: 264发表: 1982年 Quantitative studies of enzyme-substrate compartmen...