bovinus and S. luteus . The mycelium of T. harzianum turned out to be the best substrate for the induction of β-1,3-glucanases and β-glucosidases for both strains of L. laccata , although the difference in the induction of chitinases in the presence of mycelia of different species ...
Interestingly, PARP1 is hyper-ubiquitinated in response to DUB inhibition, which increases its enzymatic activity. Our study uncovers key regulatory roles of DUBs and provides a resource of endogenous ubiquitination sites to aid the analysis of substrate specific ubiquitin signalling....
“Creating a database with enzyme-substrate pairs”). For training our machine learning models, we extracted 18,351 enzyme-substrate pairs with experimental evidence for binding, comprised of 12,156 unique enzymes and 1379 unique metabolites. We also extracted 274,030 enzyme-substrate pairs with ...
Influence of Glucose and Saturated Free-Fatty Acid Mixtures on Citric Acid and Lipid Production by Yarrowia lipolytica In the present report, the effect of glucose and stearin (substrate composed by saturated free-fatty acids) on the production of biomass, reserve lipid, an... S Papanikolaou,M ...
Substrate specificity of two thrombin like enzymes (elegaxobin, elegaxobin II) from the venom of Trimeresurus elegans (Sakishima-habu), using neutralizing antibodydoi:10.1016/j.toxicon.2005.08.007We reported previously that elegaxobin and elegaxobin II cause the release of fibrinopeptide A ...
Be careful, though, as not all enzymes end in -aseand not all enzymes have the substrate they act on in their name either. For example, the amylase enzyme, shown in Figure 4, breaks down starch into maltose, which is then broken down into glucose by another enzyme called maltase. Figur...
The enzyme activities on different substrates were assayed by monitoring the reducing groups liberated by the enzymatic hydrolysis of the polymeric substrate. Reducing sugars were quantified with the colorimetric method of Nelson and Somogy (Ashwell, 1957). The formation of the arsenomolybdate–copper ...
PqsR transcriptional regulator interactions with substrate(s) and inhibitor(s). AThe chemical formula of a natural binder of the PqsR transcriptional regulator, 2-heptyl-4-hydroxyquinoline,Bthe hydroxyl group at the position-3 of 2-heptyl-4-quinolone (HHQ) has a pivotal role in PqsR and HHQ...
and we were thus unable to create a version specific to the task of predictingkcat. However, we speculated that the ESM-1b vectors fine-tuned previously for the prediction of enzyme-substrate pairs might also improvekcatpredictions. To test this hypothesis, we used our previously published model...
Challenges encompass material flexibility, electrode nanostructure, stability of enzyme and mediator immobilization, electron transport efficiency, substrate and O2 availability, and biocompatibility. One major challenge often results in low or fluctuating current outputs, such as low amperometric currents in ...