B, MALDI-TOF MS analysis of purified sulfatase digested with trypsin and CNBr (numbers indicate the amino acid residues). C, specific activity of the purified sulfatases. The specific sulfatase activity assay of each enzyme confirmed the results obtained by MALDI-TOF MS (Fig. 9C). No ...
One of the approaches to identifying specific substrates for DUBs is a yeast two-hybrid screen. Some DUBs reveal a specific interaction with their substrates regardless of the ubiquitylation state (Fig.1d) This increases the probability of finding these substrates in the yeast-two-hybrid system. Yo...
One of the approaches to identifying specific substrates for DUBs is a yeast two-hybrid screen. Some DUBs reveal a specific interaction with their substrates regardless of the ubiquitylation state (Fig.1d) This increases the probability of finding these substrates in the yeast-two-hybrid system. Yo...
Each type of enzyme has a specific three-dimensional shape that enables it to fit with the substrate, which has a complementary shape. The link between enzymes and substrates is so strong that enzymes often are named after the substrate involved, simply by adding ase to the name of the ...
Interaction of the resolving enzyme YDC2 with the four-way DNA junction. Holliday junctions (four-way DNA junctions), formed during homologous recombination, are bound and resolved by junction-specific endonucleases to yield rec... MF White,Lilley David M.J - 《Nucleic Acids Research》 被引量:...
However, in the case of enzymes which interact with small size substrates such as for example, phosphoglycerate kinase and chitobiase, only one part of the protein, presumably that related directly or indirectly to the catalytic site, appears to be flexible, whereas the other parts are even more...
Besides their specific catalytic functions all these proteins should exhibit certain common modes of protein 鈥 nucleotide interactions because the amino, imino, carbonyl, hydroxyl, and phosphate groups of nucleotides represent but a limited array of substituents capable to interact with protein residues...
p pBackground/p pUbiquitin (E3) ligases interact with specific ubiquitin conjugating (E2) enzymes to ubiquitinate particular substrate proteins. As the combination of E2 and E3 dictates the type and biological consequence of ubiquitination, it is important to understand the basis of specificity in ...
Adenylate-forming enzymes are a mechanistic superfamily that are involved in diverse biochemical pathways. They catalyze ATP-dependent activation of carboxylic acid substrates as reactive acyl adenylate (acyl-AMP) intermediates and subsequent coupling to
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