enzymessubstrate depletionproduct accumulationcoupled measurementsoptical detectionelectrochemical detectionultra-high-throughput assayspractical considerationsSummary Methods for the quantitation of enzymes and their substrates, including substrate depletion and product accumulation measurements, direct and coupled ...
In light of the results presented here, we envisage that the Glycologue HMO-enzyme simulator will be extended, or adapted, as our knowledge of the enzymes and their substrate specificities improves. The model presented here is a pathway model, which does not account for abundances of individual...
This review will attempt to summarise results from the last few years that shows that DUBs are an essential regulatory step of both protein degradation by the proteasome, and of other ubiquitin-dependent processes, by virtue of their ability to regulate protein ubiquitination in a target-specific ...
Although binding and cleavage are well understood, the question remains how the enzymes locate their substrate within long duplex DNA. Here we track fluorescent dimers of endonuclease I on DNA, presenting the complete single-molecule reaction trajectory for a junction-resolving enzyme finding and ...
Results indicate that the microsomal enzyme inducer, phenobarbital, binds to microsomes and to soluble cytochrome P 450, is readily displaced by hexobarbital (type I substrate) or aniline (type II substrate), and does not greatly inhibit the interaction of substrates with cytochrome P 450. On ...
The substrate inhibition exhibited by carboxypeptidase A in catalysing the hydrolysis of (S)-2-O-(N-benzoylglycyl)-β-phenyllactate) is limited by addition of cyclodextrins. The cyclodextrins do not significantly change the maximum rate of reaction, but they increase the concentration of the substra...
Given that the interacting regions between DUBs and their substrates (or cognate ubiquitin ligases) are now being defined, it is hoped that more rapid progress towards specific drugs will be made. For example, given the apparent primary role of UBP7 (USP7) in regulating MDM2 levels, and the...
SB155-2 and Dr0824 from Deinococcus radiodurans R1 were cloned, purified, and their substrate profiles determined. These enzymes were previously incorrectly annotated as adenosine deaminases or chlorohydrolases. It was shown here that these enzymes actually deaminate 6-aminodeoxyfutalosine. The ...
Available data on the primary and secondary specificities of chymotrypsin, trypsin, elastase and subtilisin are reviewed in relation to their structures. For chymotrypsin and trypsin, proper binding in the main specificity pocket S1 is sufficient to ensure almost perfect orientation of the substrate for...
Using substrate mimicry and reaction optimization, their function has now been repurposed to catalyse intermolecular isopeptide bond formation on diverse peptide and protein substrates. Fabian B. H. Rehm , Tristan J. Tyler & Thomas Durek Article 03 May 2024 | Open Access Selection of a ...