NMR spectroscopy can provide information on many different aspects of proteinligand interactions-not only structural but also dynamic, kinetic and thermodynamic (Jardetzky and Roberts, 1981; Feeney and Birdsall, 1993). For protein-ligand complexes of relatively modest size (<30kD), the use of ...
Assessing the Balance between Protein?Protein Interactions and Enzyme?Substrate Interactions in the Channeling of Intermediates between Polyketide Synthase... 6-Deoxyerythronolide B synthase (DEBS) is the modular polyketide synthase (PKS) that catalyzes the biosynthesis of 6-deoxyerythronolide B (6-dEB...
Between these two limits are various interactions of intermediate strength which account for the cohesion and yet mobility of liquids, and for the existence of specific biologically important complexes such as those of antibody-antigen, hormone-receptor and enzyme-substrate....
but current approaches have exclusively concentrated on addressing one of these issues, overlooking the similarity of both tasks in reflecting the relationship of protein sequences towards substrate structures. A statistical approach has been proposed to inferKmvalues across...
Activity-based protein profiling (ABPP) has been used extensively to discover and optimize selective inhibitors of enzymes. Here, we show that ABPP can also be implemented to identify the converse—small-molecule enzyme activators. Using a kinetically controlled, fluorescence polarization-ABPP assay, we...
Key enzyme–substrate interactions were identified that accounted for the exclusive regioselectivity and stereoselectivity of the enzyme. Additionally, comparison of the four CYP154C5–steroid structures revealed distinct structural differences, explaining the observed variations in kinetic data obtained for ...
(1) in which the underlying reason for the irreversible inhibition was proposed to be differences in the stereochemical arrangement at the transition states for enzyme acylation by the natural substrate and enzyme phosphonylation by the ... IM Kovach - Springer US 被引量: 50发表: 1998年 Lipid...
(Bayer, 2004; Schoffelen and van Hest2012). Compared with other co-localization techniques, protein scaffolds have some advantages. First, the minimum distance between the active site on the protein scaffold allows for effective substrate channels, because metabolic intermediates are more likely to ...
The polymeric characteristics of the PET substrate and protein–protein interactions demand extraordinary control of the orientation of the active sites of MHET hydrolase and PET hydrolase. Moreover, the complex structure spontaneously formed between KL-MHETase and FAST-PETase in a mixed dual enzyme sy...
The reduction of mutual stabilisation of the two domains on binding substrate indicates a freeing of the hinge to allow the protein to take up other states rather than a directive mechanism. The stabilisation of both domains at higher concentrations of ATP at which the enzyme is inhibited ...