AUGMENTATION OF EGFR MRNA EXPRESSION IN LUNG CANCER CELL LINE A549 DUE TO IN VITRO APICAL INFECTION WITH RESPIRATORY TRACT PATHOGENIC BACTERIA PSEUDOMONAS AERUGINOSAAl-Qaissy, Omayma SaadAl-Khafaji, Ahmed S. K.Biochemical & Cellular Archives
Total 10 NSCLC cell lines (EGFR wild type; A549, H1299, H292, EGFR mutant; PC9, Ma1, KT2, KT4, Ma47, H1975, H1650) including these four cell lines have been analyzed for the relationship between EGFR gene mutations, EGFR gene amplification, EGFR protein expression as markers for ...
Realtime fluorescence quantitative PCR( QPCR),enzyme-linked immunosorbent assay( ELISA) and Western blotting were used to detect EGFR,TGF-α gene and protein expression in A549 cells,respectively. In addition,the expression of γ-H2 AX was determined by immunofluorescence assay. Results The ...
;compared with lung adenocarcinoma,the expression levels of microRNA-21and EGFR in lung squamous cell carcinoma were significandy increased(P<0.05). Conclusion MicroRNA-21and its regulatory gene EGFR are highly expressed in NSCLC lung adenocarcinoma and squamous cell carcinoma,and the expression ...
4b). These results indicate that FBXL2 can inhibit EGFR expression, which is dependent on its E3 ubiquitin ligase activity. Fig. 1: FBXL2 targets EGFR for polyubiquitin- and proteasome-mediated degradation in an EGF-independent manner. a H1299, PC-9, or H1975 cells stably expressing ...
临床医学;外科学 指导教师:**刚 201205 ・中文论著摘要・ MiR-7可以通过对EGFR及IGFlR信号通路的抑制来 加强吉非替尼对非小细胞肺癌的细胞毒性 目的 探索上调肺腺癌A549细胞中miR.7的表达水平能否增强吉非替尼对非小细 胞肺癌的细胞毒性,观察细胞增殖率的变化及吉非替尼IC50等的变化,并探索导 致这种结果的...
Nuclear translocation of EGFR by AKT-dependent phosphorylation enhances BCRP/ABCG2 expression in gefitinib-resistant cells Epidermal growth factor receptor (EGFR), an aberrantly overexpressed or activated receptor tyrosine kinase in many cancers, plays a pivotal role in cancer progression and has been an...
lungcancer.[Methods]MiRNAarraywasperformedtoanalysisthechangesofmiRNAexpressioninlung cancercellsbeforeorafterEGFstimulation.Transwellcellmigrationandinvasionassaysandwoundhealing assaytodeterminedtoknowthefunctionofmiR-125a-5pinlungcancercellmigrationandinvasioncapa- ...
In human adenocarcinoma, claudin-2 expression is higher than that in normal lung tissue, but the regulatory mechanism of its expression has not been clarified. In human adenocarcinoma A549 cells, claudin-2 level time-dependently increased under the control conditions. In contrast, claudin-1 expressio...
Gene expression profiles in asbestos-exposed epithelial and mesothelial lung cell lines We exposed the human cell lines A549, Beas-2B and Met5A to crocidolite asbestos and determined time-dependent gene expression profiles by using Affymetrix... Penny,Nymark,Pamela,... - 《Bmc Genomics》 被引量...