6). Consistently, PYR1/PYL4 and PYL8 have been shown to be degraded by a single subunit RING-type E3 ubiquitin ligase RSL1 (RING FINGER OF SEED LONGEVITY1) and a DDA1 (DET1-, DDB1-ASSOCIATED1) E3 ligase, respectively36,37. To determine whether ABI1 can be ubiquitinated in plant ...
It is possible that interaction of ABI1 with PYLs changes the conformation of ABI1, which may create a suitable surface for ubiquitin transfer. Although the degradation of ABI1 in a PYL quadruple mutant is largely reduced (Fig. 5), ABI1 protein level is lower in the PYL quadruple mutant ...
The first step is an ATP-dependent activation of ubiquitin (UBQ) by an E1 enzyme. The E1 binds the UBQ and transfers it in a second step to an UBQ-conjugating E2 enzyme. In the third step the E2 physically associates with an E3 UBQ ligase that has recruited a substrate protein to ...