FLDS: A comprehensive dsRNA sequencing method for intracellular RNA virus surveillance. Microbes Environ. 31:33-40.Urayama S, Takaki Y, Nunoura T (2016) FLDS: a comprehensive dsRNA sequencing method for intracellular RNA virus surveillance. Microbes Environ 31:33–40 Central...
[6]Singh, S., Gupta, M., Pandher, S., Kaur, G., Goel, N., Rathore, P., et al. (2019b). RNA sequencing, selection of reference genes and demonstration of feeding RNAi in Thrips tabaci (Lind.) (Thysanoptera: Thripidae). BMC Mol. Biol.20:6. doi: 10.1186/s12867-019-0123-1 [...
已经自寄生线虫例如Meliodogyne和异皮线虫属物种中克隆了这样的基因,或者本领域技术人员可使用来自克隆的秀丽隐杆线虫(C.elegans)直向同源物的序列信息来鉴定这样的基因(秀丽隐杆线虫基因组已被测序并且是可用的,参见秀丽隐杆线虫测序协会(TheC.elegansSequencingConsortium)(1998))。若干实施方案涉及将病原体抗性赋予...
Sequencing was performed with an ABI Prism BigDye Terminator v 3.1 cycle sequencing kit (Applied Biosystems) and 5 pmol of the sequencing primers provided with the EZ-Tn5™ kit on an ABI-3730xl automated DNA analyzer (Applied Biosystems) according to the manufacturer’s manual. The bidirection...
These results show that sRNA sequencing is useful to compare exogenous RNAi delivery methods on peanut plants, and to analyze the efficacy of molecular constructs to generate siRNAs against specific gene targets. This work lays the foundation for non-transgenic delivery of RNAi in controlling ...
Deep sequencing was performed to analyze the biogenesis of siRNA populations. Although long-dsRNA remained in the treated leaves for at least 10 days, its systemic movement was not observed. Conversely, dsRNA-derived siRNA populations (mainly 21- and 22-nt) were detected in non-treated leaves, ...
RNA isolated from human foreskin keratinocytes with or without Poly (I:C) (30 µg per ml) (GSE92646) and mouse wound tissues from wild type (C57BL/6NJ) and Tlr3−/− with BMS493 and RA were submitted to the JHMI Deep Sequencing & Microarray core for Affymetrix® Human Exon ...
Development of novel sequencing strategies and phylogenetic analysis of the orbiviruses using this database has identified five novel Orbivirus species and has facilitated development of a pan-orbivirus RT-PCR assay that can be used to identify the RNA of any Orbivirus species. These techniques ...
MolecularCloningand Sequencing of DNA Encoding VP_2 structural Protem on Attenuated strain of Muscovy Duck Parvovirus; 番鸭细小病毒弱毒株VP_2基因的分子克隆与序列测定 3) attenuated vaccine 弱毒疫苗 1. Comparison of immune response in BALB/c mice after immunization with GPV-VP3 gene vaccine oratten...
dsRNA新序列及dsRNA 新病毒的发现及研究,为dsRNA病毒种类和数量的研究积累信息。本论文对报春花和某 链格孢属真菌B中的dsRNA进行了序列研究和功能分析。 从杭州地区采集有黄边症状的栽培观赏性植物报春花,通过从叶组织中抽提dsRNA 并进行1%琼脂糖电泳,检测出大小约2300bp的dsRNA条带。利用经修改的单引物扩增 法(...