网络足迹实验 网络释义 1. 足迹实验 4.7 DNA 酶I足迹实验(DNase I footprinting assays)93-95 cdmd.cnki.com.cn|基于 1 个网页
Applications Preparation of DNA-free RNA samples Removal of DNA contamination from RNA samples prior to RT-qPCR reaction DNA template after removal of RNA transcription in vitro DNase I footprinting assay Construction of DNA random fragment library...
The DNase I footprinting method was first described by Galas and Schmitz (1). The technique remains unsurpassed as a way of gaining direct and immediate information about the location of a protein binding site in the DNA sequence. In addition, the footprinting method provides a way of studying...
DNase I footprinting assays together with Transfac analyses suggest that the mosquito LCH promoter contains putative GATA, E2F, NIT2, TATA and DPE sites, and that one of the HCH-regulatory elements can alter the expression of the A. aegypti ferritin LCH gene. For many years, the prevailing ...
Gel retardation and DNase I footprinting assays further reveal that the H2A modulator contains at least two binding sites [upstream sequence elements 1 and ... F Palla,C Casano,I Albanese,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 37...
图1. 脱氧核酸糖酸酶I(DNase I)作用机理图 主要应用: 制备不含DNA的RNA样本; RT-PCR反应前RNA样本内基因组DNA等可能存在DNA污染的去除; T7,T3,SP6等RNA聚合酶催化的体外RNA转录后DNA模板的去除; DNase I足迹法(DNase I footprinting)研究DNA-蛋白质相互作用; ...
I Barozzi,P Bora,MJ Morelli 摘要: DNase I is an enzyme preferentially cleaving DNA in highly accessible regions. Recently, Next-Generation Sequencing has been applied to DNase I assays (DNase-seq) to obtain genome-wide maps of these accessible chromatin regions. With high-depth sequencing, ...
3. Identification of protein binding sequences on DNA (DNase I footprinting), 4. Prevention of clumping when handling cultured cells, and 5. Creation of a fragmented library of DNA sequences for in vitro recombination reactions. While frequently used in the laboratory, the...
footprintinggene regulatory networksDNase I is an enzyme preferentially cleaving DNA in highly accessible regions. Recently, Next-Generation Sequencing has been applied to DNase I assays (DNase-seq) to obtain genome-wide maps of these accessible chromatin regions. With high-depth sequencing, DNase I ...
RNase-Free DNase I 产品说明书 Manual RNase-Free DNase I manual For Research Use Only. Not for use in diagnostic procedures.RNase-Free DNase I is part of the Epicentre™ product line,known for its unique genomics kits, enzymes, and reagents which offer high quality and reliable performance.