DNase I footprinting - Leblanc, Moss - 1994 () Citation Context ... 0?1 mM EDTA) in a total volume of 10 ml. DNase I (0?01 Kunitz units, Fermentas) was added to each sample. Ten microlitres of stop buffer (100 mg yeast tRNA ml 21 , 30 mM EDTA, 1 % SDS, 200 mM NaCl) (...
DNase I footprinting was developed by Galas and Schmitz in 1978 as a method to study the sequence-specific binding of proteins to DNA (1). In this technique a suitable ...
DNase I footprinting Nick Translation 相关产品 单独销售的组分 DNase I 反应缓冲液 注意事项 EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation (3). We do not recommend using NEB's DNase I as a substitute for Monarch DNase ...
DNase I footprinting was first published in 1978 and predates both Sanger sequencing and NGS. The first published use with NGS was published by Boyle et al. and later optimized for sequencing1. A high-sensitivity protocol is also available (scDNase-seq)2. ...
RNase-Free DNase I 产品说明书 Manual RNase-Free DNase I manual For Research Use Only. Not for use in diagnostic procedures.RNase-Free DNase I is part of the Epicentre™ product line,known for its unique genomics kits, enzymes, and reagents which offer high quality and reliable performance.
DNase I footprinting was first published in 1978 (Galas et al., 1978) and predates both Sanger sequencing and NGS. The first published use with NGS was published by Boyle et al. 2008 (Boyle et al., 2008) and later optimized for sequencing (Anderson et al., 1981). A high-sensitivity ...
1. Degradation of contaminating DNA after RNA isolation, 2. "Clean-up" of RNA prior to RT-PCR and after in vitro transcription, 3. Identification of protein binding sequences on DNA (DNase I footprinting), 4. Prevention of clumping when handling cultured cells, and ...
DNase Dnase 1 Dnase 1 Dnase 1 DNase footprinting assay DNase I DNase I DNase I DNase II DNase II-like acid DNase DNase IIbeta DLAD DNase IV DNASOA DNAT Dnata DNAU DNAV DNAWS DNAX-activating protein of 10KDa DNB DNB DNB DNB5 DNBA DNBAM DNBAR DNBB DNBBS DNBC DNBCC DNBCD ▼Complete...
Dnase 1 Dnase 1 DNase footprinting assay DNase I DNase II DNase II-like acid DNase DNase IIbeta DLAD DNase IV DNASOA DNAT Dnata DNAU DNAV DNAWS DNAX-activating protein of 10KDa DNAzyme DNB DNB DNB DNB5 DNBA DNBAM DNBAR DNBB DNBBS DNBC DNBCC DNBCD DNBD DNBF DNBH DNBHE DNBI DNBK...
• DNA labeling by nick-translation in conjunction with DNA Polymerase I (1), see protocol on reverse page. • Studies of DNA-protein interactions by DNase I, RNase-free footprinting (1). • Generation of a library of randomly overlapping DNA 2+ inserts. Reaction buffer containing Mn ...