Genetic footprinting has been applied to three model systems: the supF tRNA, the TEM-1 beta-lactamase protein, and the first 1000 nucleotides of the HIV-1 genome. In each case, the density of mutants analyzed has greatly exceeded that found in previous studies of that DNA segment. These ...
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The biological processes that drive cellular function can be represented by a complex network of interactions between regulators (transcription factors) and their targets (genes). A cell’s epigenetic state plays an important role in mediating these inte
binding activity (CBF2) as determined by electrophoretic mobility shift analyses, competition binding analyses, and DNase I footprinting; and (iii) a single C → T transition mutation at this site is associated with a marked reduction (> 50-fold) of transcriptional activity in a reporter plasmid...
Regulatory elements play an important role in the variability of individual responses to drug treatment. This has been established through studies on three classes of elements that regulate RNA and protein abundance: promoters, enhancers and microRNAs. E
Mobile group II introns are bacterial retrotransposons that combine the activities of an autocatalytic intron RNA (a ribozyme) and an intron-encoded reverse transcriptase to insert site-specifically into DNA. They recognize DNA target sites largely by ba
However, sequences of the promoter/enhancer complex failed to compete for its . -copper ion footprinting experiments showed that the sites for the sunflower proteins in HaG3A (-1463 to -1514 and -702 to -653) and in phaseolin (-671 to -627) are also very A/T-rich, have similar ...
In eukaryotic cells, chromatin is a basic hereditary unit, which consists of DNA, histone proteins and other genetic materials, and regulates cell type-specific gene expression [1,2]. Chromatin, as a dynamic nuclear structure, is transcriptionally active in the interphase, and is relatively inactiv...
2.1. Analytical Platforms One of the primary difficulties facing the development of metabolomics has been the staggering diversity of metabolites. Metabolites are substantially more chemically diverse than the subunit-based chemistries of DNA, RNA, and proteins, impeding the progress of metabolomics as a...
RNA-Grade Ribonucleases:A collection of ribonucleases (RNases AandT1) that are optimized for researchers performing RNA structure, RNA sequencing, protein footprinting and boundary experiments. Poly(A)Polymerase:Catalyzes the addition of adenosine to the 3' end of RNA in...