DNase I Footprinting精确鉴定调控蛋白在DNA上的结合序列服务简介DNase I footprinting assay(DNase I足迹分析实验)可以精确鉴定DNA结合蛋白(如转录调控蛋白)在DNA分子上的结合位点。转录调控蛋白通过与启动子区域结合来调控靶标基因的转录。目前,凝胶阻滞实验(EMSA)和DNase I足迹分析实验是重要的一套体外研究工具:凝胶...
B. (1994). Solid phase DNase I footprinting: quick and versatile. Nucleic Acids Res. 22:1511-1512.Brenowitz M, Senear DF, Kingston RE 2001. DNase I footprint analysis of protein-DNA binding . Curr Protoc Mol Biol Chapter 12:Unit 12.4. doi:.10.1002/0471142727.mb1204s07 [ Cross Ref ]...
Quantitative Methods of Analysis of Footprinting Diagrams for the Complexes Formed by a Ligand with a DNA Fragment of Known Sequence The regulation of gene expression is based on the interaction of DNA with different ligands. A model of adsorption was considered that can be applied to th... YD...
DNAse footprinting: a simple Shinozaki K, Seki M. Arabidopsis transcriptome analysis method for the detection of protein-DNA binding specific- under drought, cold, high-salinity and ABA treatment ity. Nucleic Acids Res , 1978, 5(9): 3157–3170. DOI conditions using a tiling array. Plant Cell...
DNase I footprinting was developed by Galas and Schmitz in 1978 as a method to study the sequence-specific binding of proteins to DNA (1). In this technique a suitable uniquely end-labeled DNA fragment is allowed to interact with a given DNA-binding protein and then the complex is partially...
Dnase 1 DNase footprinting assay DNase I DNase II DNase II-like acid DNase DNase IIbeta DLAD DNase IV DNASOA DNAT Dnata DNAU DNAV DNAWS DNAX-activating protein of 10KDa DNAzyme DNB DNB DNB DNB5 DNBA DNBAM DNBAR DNBB DNBBS DNBC DNBCC DNBCD DNBD DNBF DNBH DNBHE DNBI DNBK ▼Comple...
用途:制备不含DNA的RNA样品;RT-PCR反应前RNA样品中去除基因组DNA等可能的DNA污染;体外T7, T3, SP6等RNA Polymerases催化的RNA转录后去除DNA模板; DNase I footprinting研究DNA-蛋白质相互作用;缺口平移(nick translatioin);产生DNA随机片断文库;细胞凋亡TUNEL检测中部分剪切基因组DNA作为阳性对照。
Protein Analysis Nucleic Acid-Protein Interactions DNA-Protein Interactions DNase I Footprinting Assay Service Profacgenprovides DNase I footprinting assay service for the detection ofDNA-protein interactionandidentification of the exact binding sitesof DNA-binding proteins. DNase I footprinting assay is bas...
Computational footprinting methods for next-generation sequencing experiments English) Transcriptional regulation orchestrates the proper temporal and spatial expression of genes. The identification of transcriptional regulatory elem... E Gusmo,S Decker,M Zenke,... 被引量: 0发表: 2017年 Next Generation ...
DNase I footprinting assays together with Transfac analyses suggest that the mosquito LCH promoter contains putative GATA, E2F, NIT2, TATA and DPE sites, and that one of the HCH-regulatory elements can alter the expression of the A. aegypti ferritin LCH gene. For many years, the prevailing ...