DNase I footprinting Kit 产品性能报告 (IEMed-K324) 实验目的: 转录因子在靶基因上的结合位点研究; 技术原理: DNase I足迹(DNase I footprinting)实验是一种鉴别核酸结合蛋白质在DNA上结合位点的经典方法,它能明确核酸结合蛋白结合的碱基位置。该技术的基本原理是蛋白质结合DNA片段后,能保护结合区域核苷酸不被DNase...
DNase I footprinting研究DNA-蛋白质相互作用; 缺口平移(nick translatioin);产生DNA随机片断文库; 细胞凋亡TUNEL检测中部分剪切基因组DNA作为阳性对照。37℃,10分钟内,将能够完全降解1μg pBR322质粒DNA所需的酶量定义为1个活性单位。大肠杆菌表达的31kd重组蛋白。 组分 规格 RNase-free DNase I(3U/μL) 0.5mL...
DNase I footprinting Nick Translation 相关产品 单独销售的组分 DNase I 反应缓冲液 注意事项 EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation (3). We do not recommend using NEB's DNase I as a substitute for Monarch DNase ...
PCR纯化试剂盒/DNA纯化试剂盒价格:￥151/支产品详情:PCR纯化试剂盒/DNA纯化试剂盒沪震生物的PCR纯化试剂盒(PCR Clean Up Kit),也称DNA纯化试剂盒(DNA Purification Kit)是一种用于P... 纯度:99% 包装规格:50次 用途:科研使用 货号:HZ0033 品牌:沪震生物 ...
包括制备不含DNA的RNA样品;RT-PCR反应前RNA样品中去除基因组DNA等可能的DNA污染;体外T7, T3, SP6等RNA Polymerases催化的RNA转录后去除DNA模板; DNase I footprinting研究DNA-蛋白质相互作用;缺口平移(nick translatioin);产生DNA随机片断文库;细胞凋亡TUNEL检测中部分剪切基因组DNA作为阳性对照等。
Dnase 1 DNase footprinting assay DNase I DNase I DNase I DNase II DNase II-like acid DNase DNase IIbeta DLAD DNase IV DNASOA DNAT Dnata DNAU DNAV DNAWS DNAX-activating protein of 10KDa DNB DNB DNB DNB5 DNBA DNBAM DNBAR DNBB DNBBS DNBC DNBCC DNBCD ▼Complete...
DNase I footprinting was first published in 1978 and predates both Sanger sequencing and NGS. The first published use with NGS was published by Boyle et al. and later optimized for sequencing1. A high-sensitivity protocol is also available (scDNase-seq)2. ...
• DNA labeling by nick-translation in conjunction with DNA Polymerase I • Studies of DNA-protein interactions by DNase I, RNase-free footprinting • Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used ...
• Studies of DNA-protein interactions by DNase I, RNase-free footprinting • Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used Note DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex. ...
5. Used in Footprinting method to analyze DNA-protein interaction.6. DNA fragment library construction in the presence of Mn2. Product Components Product Components Cat. No. /Size DNI-DL001-B (1000U) DNI-DL001-C (5000U) DNase I (RNase-free, 4U/μl) 250μl 1.25ml Other Notes 1. ...