LCK inhibition attenuates HR repair efficiency The inhibition of DNA damage repair genes led us to test whether LCK inhibition impairs HR-dependent DNA repair. We utilized the DR-GFP reporter assay established in U2OS cells to measure repair efficiency [25] (Fig. 4A). U2OS cells with/without ...
We suggest that de novo DNA methylation at the site of DSB repair stabilizes the H3K9m3 mark, which alters the chromatin structure of the entire GFP gene. To map specific domains modified by DNA damage and repair, we examined the structure of chromatin at the repaired locus in UnRec (repair...
Interaction between endogenous ABRO1 and endogenous p53, examined by Co-IP assay, was observed in HCT116 p53+/+ cells (Fig. 6a) but not in HCT116 p53−/− cells. Consistent with the results showing that DNA damage induced ABRO1 upregulation and nuclear translocation, the interaction ...
Genetics & Pharmaceutical,Population Health Management, Nutrition and Phytochemistry,Precision Cancer Medicine,Prostate Cancer: Monitoring vs Treatment,RNA Biology, Cancer and Therapeutics,tumor microenvironment, taggedCancer,DNA damage,DNA repair,gender,mutation,sexon April 2, 2019|Leave a Comment » Gende...
DNA damage, especially DNA double strand breaks (DSBs) and replication stress, activates a complex post-translational network termed DNA damage response (DDR). Our review focuses on three PI3-kinase related protein kinases—ATM, ATR and DNA-PKcs, which s
Qiao Y, Spitz MR, Guo Z, Hadeyati M, Grossman L, Kraemer KH, Wei Q (2002) Rapid assessment of repair of ultraviolet DNA damage with a modified host-cell reactivation assay using a luciferase reporter gene and correlation with polymorphisms of DNA repair genes in normal human lymphocytes. ...
当然,这只是DNA众多修复方式中的一个,并非能100%完成修复,这也一定程度上解释了文章的结果errorbar比较大,猜测assaywindow和稳定性都不是很好把控。 为了构造类似的修复方式观察Polθ的修复能力,文中选择了Nanoluciferase作为报告基因,可能利用其灵敏性较高的特点,结合SV40转录终止子和CMV启动子,构建了MMEJ的底物。
The purpose of this study was to determine whether the ability of K-1735 murine melanoma cells to repair DNA damage correlates with their metastatic potential. Three nonmetastatic clones, four metastatic clones, and three somatic-cell hybrids between metastatic and nonmetastatic clones were exposed to...
To further corroborate this result, we continued to assess the DNA repair efficiency of neurons using a previously described NHEJ reporter assay12. We found that, compared with cells infected with Scr-shRNAs, knockdown of BS-DRL1 dramatically reduced the NHEJ-mediated DSB repair (Fig. 5n). We...
M. et al. The human DEK oncogene regulates DNA damage response signaling and repair. Nucleic acids research 39, 7465–7476, doi: 10.1093/nar/gkr454 (2011). 20. Wise-Draper, T. M. et al. Overexpression of the cellular DEK protein promotes epithelial transformation in vitro and in vivo. ...