其中,该研究使用了DNA亲和纯化测序技术(DAP-seq,DNA Affinity Purification Sequencing),鉴定了水稻生物钟核心组分OsCCA1(Oryza sativa CIRCADIAN CLOCK ASSOCIATED 1)调控的下游靶基因。 2020年4月,北京工商大学与蓝景科信合作,Biochemical and Biophysical Research Communications(IF=3.322)发表了题为“ Phytochrome-interact...
DNA affinity purification sequencing (DAP-seq) couples in vitro expression of transcription factor (TF) and binding to a genomic DNA library with next-generation sequencing, providing a robust method to identify genome-wide transcription factor binding sites (TFBS) on endogenous genomic DNA. Here we...
背景: DAP-seq: DNA affinity purification sequencing(DNA亲和纯化测序) AUXIN RESPONSE FACTORS (ARFs):plant-specifictranscription factors (TFs) that couple perception of the hormone auxin to geneexpression programs. highly conserved nuclear auxinsignal transduction pathway is composed of the TIR1/AFB-Aux/...
为了探究LFY-UFO基因组结合位点,作者通过ampDAP-seq(amplified DNA affinity purification sequencing),发现600多个UFO-LFY特异的结合位点,表明UFO和LFY形成复合体,识别新的DNA顺式作用元件,调控基因表达(图1)。 图1 LFY和UFO协同激活AP3表达 此外,作者发现,LFYK249R突变不影响LFY原本活性,但抑制UFO-LFY与下游靶...
进一步研究发现,LFY-UFO激活AP3转录的结合位点不同于经典的LFY结合位点。为了探究LFY-UFO基因组结合位点,作者通过ampDAP-seq (amplified DNA affinity purification sequencing) ,发现600多个UFO-LFY特异的结合位点,表明UFO和LFY形成复合体,识别新的DNA顺式作用元件,调控基因表达 (图1) 。
(PCLs), one family of PRC2-associated factors23, to specific DNA sequence, which may affect transcription states, higher-order chromatin interactions and chromatin states24,25. A high-throughput TF-binding site discovery method, namely DNA affinity purification sequencing (DAP-seq), allows to ...
[Part 1: Biological Sciences] || Affinity Purification of Bacteriophage T4 Proteins Essential for DNA Replication and Genetic Recombination T Formosa,BBM Alberts - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 0发表: 1983年 Affinity purification of ...
Sequencing of dOG in this context also allowed us to demonstrate the feasibility of using dMMO2ssbioTP for affinity purification of the labelled strands. The dOG-containing plasmid was subjected to a one-pot replacement of the lesion by the UBP via the method outlined above. Before Sanger ...
DNA purification and isolation using magnetic part 优质文献 相似文献 参考文献 引证文献Isolation of Xis Gen Fragment of λ Phage from Agarose Gel Using Magnetic Particles for Subsequent Enzymatic DNA Sequencing DNApurificationMagneticparticlesGel electrophoresis is one of the most important methods used in...
Purification Affinity Chromatography Applications WB, ICC, IHC-P, IHC-F, ELISA Concentration 500ug/ml UOM 200ug Conjugate No Conjugate ANTIBODY SPECIFITY DNA指导聚合酶β(POLβ)单克隆抗体The antibody is a mouse monoclonal antibody raised against POLb. It has been selected for its ability to recogni...